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Enhanced production of protease by Pseudoalteromonas arctica PAMC 21717 via statistical optimization of mineral components and fed-batch fermentation

Cited 4 time in scopus
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Title
Enhanced production of protease by Pseudoalteromonas arctica PAMC 21717 via statistical optimization of mineral components and fed-batch fermentation
Other Titles
미네랄 조성의 통계적 최적화와 유가식 발효를 통한 Psedoalteromonas arctica PAMC 21717에 의한 단백질분해효소 생산의 증대
Authors
Han, Se Jong
Yim, Joung Han
Park, Ha Ju
Kim, Dockyu
김성희
Park, Heeyong
Keywords
Pseudoalteromonas arctica PAMC 21717; Psychrophile; Statistical optimization
Issue Date
2016
Citation
Han, Se Jong, et al. 2016. "Enhanced production of protease by Pseudoalteromonas arctica PAMC 21717 via statistical optimization of mineral components and fed-batch fermentation". PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 46(4): 328-335.
Abstract
The objective of this study was to statistically optimize the mineral components of the nutritional medium required for enhancing the production of a cold-active extracellular serine-type protease, W-Pro21717, by the Antarctic bacterium Pseudoalteromonas arctica PAMC 21717. Skim milk was identified as the major efficient inducer. Among the 12 components included in the unoptimized medium, skim milk, NaCl, Na2SO4, Fe(C6H5O7) (ferric citrate), and KCl were determined, by the Plackett-Burman and Box-Behnken design, to have a major effect on W-Pro21717 production. Fed-batch fermentation (5 L scale) using the mineral-optimized medium supplemented with concentrated skim milk (critical medium component) resulted in a W-Pro21717 activity of 53.4 U/L, a 15-fold increment in production over that obtained using unoptimized flask culture conditions. These findings could be applied to scale-up the production of cold-active protease.
URI
http://repository.kopri.re.kr/handle/201206/7340
DOI
http://dx.doi.org/10.1080/10826068.2015.1031390
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