KOPRI Repository

A transcriptomic analysis of the response of the arctic pteropod Limacina helicina to carbon dioxide-driven seawater acidification

Cited 7 time in scopus
Metadata Downloads
Title
A transcriptomic analysis of the response of the arctic pteropod Limacina helicina to carbon dioxide-driven seawater acidification
Other Titles
이산화탄소에 의해 유도된 해양산성화에 대한 북극 익족류 리마시나 헬리시나의 전사체 발현 연구
Authors
Koh, Hye Yeon
Lee, Sung Gu
Shin, Seung Chul
Park, Hyun
Han, Se Jong
Lee, Jun Hyuck
Keywords
Arctic; Limacina helicina; biomineralization; carbon dioxide; ocean acidification; pteropod; transcriptome
Issue Date
2015
Citation
Koh, Hye Yeon, et al. 2015. "A transcriptomic analysis of the response of the arctic pteropod Limacina helicina to carbon dioxide-driven seawater acidification". POLAR BIOLOGY, 38(10): 1727-1740.
Abstract
Ocean acidification from the uptake of anthropogenic carbon dioxide (CO2) is regarded as a critical threat particularly to marine calcifying organisms. The arctic pteropod Limacina helicina may be one of the first polar organisms that are expected to display early sensitivity to ocean acidification, but a molecular approach as a foundation for understanding the effect of ocean acidification on this pteropod has rarely been reported. In this study, we examined the sub-lethal effects of CO2-driven sea-water acidification at the transcriptome level in L. helicina. cDNAs, treated under control (pH 8.2), high-CO2 (pH 7.5), and extreme-CO2 (pH?6.5) conditions, generated a total of 31,999,474 reads, comprising a total of 2,271,962,654 bp, using the Illumina platform. De novo assembly yielded 53,121 transcripts comprising 31.79 Mbp. Among the upregulated genes, 346 (0.7%) and 655 (1.2%) genes responded to extreme-level CO2 (pH 6.5) and high-level CO2 (pH 7.5), respectively. Also, 76 (0.1%) transcripts were commonly upregulated in both conditions. Among the downregulated genes, 690 (1.3%) and 739 (1.4%) genes were in response to extreme-level CO2 and high-level CO2, respectively. Also, 270 downregulated genes (0.5%) were affected in both acidic stress conditions. Moreover, 504 transcripts (1%) of biomineralization-related genes were identified;16 of these genes showed differential expression in response to acidified seawater. The dataset provides the first comprehensive overview of changes in transcript levels in the arctic pteropod L. helicina in response to increased CO2, emphasizing the potential impact of future environmental change and ocean acidification on Arctic species with external calcified structures.
URI
http://repository.kopri.re.kr/handle/201206/7347
DOI
http://dx.doi.org/10.1007/s00300-015-1738-4
Files in This Item
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse