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Application of diethyl ethoxymethylenemalonate (DEEMM) derivatization for monitoring of lysine decarboxylase activity

Cited 32 time in wos
Cited 34 time in scopus

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dc.contributor.author김용현-
dc.contributor.author박경문-
dc.contributor.authorYang, Yung-Hun-
dc.contributor.authorLee, Yoo Kyung-
dc.contributor.authorKim, Yun-Gon-
dc.contributor.authorSeo, Hyung Min-
dc.contributor.authorShashi Kant Bhatia-
dc.contributor.author신지현-
dc.contributor.author김현중-
dc.date.accessioned2018-03-29T06:06:26Z-
dc.date.available2018-03-29T06:06:26Z-
dc.date.issued2015-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/7366-
dc.description.abstractAliphatic diamines have been used as chemical reagents for the monomers of polyamides (i.e., Nylon46, Nylon 510, Nylon 66, Nylon 610 and so on), and can be made from lysine, arginine or ornithine bythe metabolism of microbes through decarboxylases. However, the conventional derivatization meth-ods for the HPLC-based quantitative analysis of aliphatic diamines exhibit poor sensitivity and efficiencyfor the monitoring of enzyme activity. In this study, a chemical derivatization method using diethylethoxymethylenemalonate (DEEMM) was applied to monitor lysine decarboxylase activity for the pro-duction of cadaverine by measuring the levels of intracellular and secreted lysine and cadaverine. Thecalibration graphs for the determination of 2,4-diaminobutyrate, 2,6-diaminopimelic acid, ornithine,arginine, lysine, 1,3-diaminopropane, putrescine dihydrochloride, cadaverine, hexamethylenediamine,and 1,7-diaminoheptane were measured by the highly sensitive method using DEEMM, and a linear rela-tionship was observed for each dimaine compound, with limits of detection up to 0.001 mM. Applicationof this method will be useful for the sensitive monitoring of lysine decarboxylase reactions throughthe detection of substrates containing amine groups and products with diamines using HPLC and a UVdetector.-
dc.languageEnglish-
dc.titleApplication of diethyl ethoxymethylenemalonate (DEEMM) derivatization for monitoring of lysine decarboxylase activity-
dc.title.alternative라이신탈탄산효소 활성 모니터링을 위한 DEEMM 활용-
dc.typeArticle-
dc.identifier.bibliographicCitation김용현, et al. 2015. "Application of diethyl ethoxymethylenemalonate (DEEMM) derivatization for monitoring of lysine decarboxylase activity". <em>JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC</em>, 115: 151-154.-
dc.citation.titleJOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC-
dc.citation.volume115-
dc.identifier.doi10.1016/j.molcatb.2015.01.018-
dc.citation.startPage151-
dc.citation.endPage154-
dc.description.articleClassificationSCI-
dc.description.jcrRateJCR 2013:53.608247422680414-
dc.subject.keywordCadevarine-
dc.subject.keywordCorynebacterium glutamicuma-
dc.subject.keywordDiamine analysis-
dc.subject.keywordDiethyl ethoxymethylenemalonate-
dc.identifier.localId2015-0122-
dc.identifier.scopusid2-s2.0-84924420170-
dc.identifier.wosid000353599300021-
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2014-2016, Environmental Change Studies Based on The Arctic Dasan Station: in terms of Geology, Atmospheric Science, and Ecology (14-16) / Lee; Yookyung (PE14030; PE15030; PE16030)
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