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Starch based polyhydroxybutyrate production in engineered Escherichia coli

Cited 12 time in scopus
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Title
Starch based polyhydroxybutyrate production in engineered Escherichia coli
Other Titles
재조합 대장균에서 polyhydroxybutyrate 생산
Authors
Shashi Kant Bhatia
Yang, Yung-Hun
Lee, Yoo Kyung
Yi, Da-Hye
Kim, Jung-Ho
이주희
Seo, Hyung Min
김현중
김용현
Christopher J. Brigham
전종민
심영하
Keywords
Amylase; Biodegradable; Panibacillus sp.; Polyhydroxybutyrate; Ralstonia eutropha
Issue Date
2015
Citation
Shashi Kant Bhatia, et al. 2015. "Starch based polyhydroxybutyrate production in engineered Escherichia coli". BIOPROCESS AND BIOSYSTEMS ENGINEERING, 38(8): 1479-1484.
Abstract
Every year, the amount of chemosynthetic plastic accumulating in the environment is increasing, and significant time is required for decomposition. Bio-based, biodegradable plastic is a promising alternative, but its production is not yet a cost effective process. Decreasing the production cost of polyhydroxyalkanoate by utilizing renewable carbon sources for biosynthesis is an important aspect of commercializing this biodegradable polymer. An Escherichia coli strain that expresses a functional amylase and accumulate polyhydroxybutyrate (PHB), was constructed using different plasmids containing the amylase gene of Panibacillus sp. and PHB synthesis genes from Ralstonia eutropha. This engineered strain can utilize starch as the sole carbon source. The maximum PHB production (1.24 g/L) was obtained with 2 % (w/v) starch in M9 media containing 0.15 % (w/v) yeast extract and 10 mM glycine betaine. The engineered E. coli SKB99 strain can accumulate intracellular PHB up to 57.4 % of cell dry mass.
URI
http://repository.kopri.re.kr/handle/201206/7368
DOI
http://dx.doi.org/10.1007/s00449-015-1390-y
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