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Controlled dehydration improves the diffraction quality of two RNA crystals

Cited 1 time in scopus
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Title
Controlled dehydration improves the diffraction quality of two RNA crystals
Other Titles
RNA 구조분석에 있어서 결정의 수분 제거를 통한 X-선 회절 향상
Authors
HaJeung Park
Matthew D. Disney
Park, Hyun
Lee, Jun Hyuck
Tuan Tran
Keywords
Post-crystallization modification; RNA; crystal dehydration; free mounting system; repeat expansion
Issue Date
2016
Citation
HaJeung Park, et al. 2016. "Controlled dehydration improves the diffraction quality of two RNA crystals". BMC STRUCTURAL BIOLOGY, 16(1): 19-NaN.
Abstract
Post-crystallization dehydration methods, applying either vapor diffusion or humidity control devices, have been widely used to improve the diffraction quality of protein crystals. Despite the fact that RNA crystals tend to diffract poorly, there is a dearth of reports on the application of dehydration methods to improve the diffraction quality of RNA crystals. We use dehydration techniques with a Free Mounting System (FMS, a humidity control device) to recover the poor diffraction quality of RNA crystals. These approaches were applied to RNA constructs that model various RNA-mediated repeat expansion disorders. The method we describe herein could serve as a general tool to improve diffraction quality of RNA crystals to facilitate structure determinations.
URI
http://repository.kopri.re.kr/handle/201206/7499
DOI
http://dx.doi.org/10.1186/s12900-016-0069-1
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