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Molecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027

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Title
Molecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027
Other Titles
해양미생물 Vibrio sp. 98CJ11027에서 특이한 Chitinase 유전자의 분리
Authors
Lee, Yoo Kyung
Lee, Hong Kum
이재형
Keywords
Vibrio sp. 98CJ11027; chitinase; glycoside hydrolase; inverse PCR cloning; marine bacterium
Issue Date
2004
Citation
Lee, Yoo Kyung, Lee, Hong Kum, 이재형. 2004. Molecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027. 2004 International Meeting of the Microbiological Society of Korea. Choenan Sangnok Convention center. 2004.05.13~14.
Abstract
The chiK gene encoding chitinase ChiK was isolated from a marine bacterium, Vibrio sp. 98CJ11027. We amplified a partial sequence of the chiK using chitinase-specific primer sets. Using inverse PCR cloning method with two specific sets of PCR primers rather than single set, we identified the 4 kb DNA fragment containing chiK gene. From the fragment, we amplified the full chiK gene and cloned into pUC18 vector designated as pChiK. The recombinant E. coli clones harboring pChiK produced a clear zone around the colony in the colloidal chitin plates. Open reading frame of the chiK is 2553 bplong encoding a ChiK precursor protein of 851 amino acids, which consist of N-terminal signal peptide of 21 amino acids and a mature ChiK protein with 830 amino acids. The deduced amino acid sequences of chiK show 89% identity with that of Vibrio carchariae chitinase. The conserved module of Vibrio sp. 98CJ11027 ChiK indicates that the ChiK belongs to the family 18 of glycoside hydrolases.
URI
http://repository.kopri.re.kr/handle/201206/7523
Conference Name
2004 International Meeting of the Microbiological Society of Korea
Conference Place
Choenan Sangnok Convention center
Conference Date
2004.05.13~14
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