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Molecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027

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dc.contributor.authorLee, Yoo Kyung-
dc.contributor.authorLee, Hong Kum-
dc.contributor.author이재형-
dc.date.accessioned2018-04-04T06:27:55Z-
dc.date.available2018-04-04T06:27:55Z-
dc.date.issued2004-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/7523-
dc.description.abstractThe chiK gene encoding chitinase ChiK was isolated from a marine bacterium, <em>Vibrio</em> sp. 98CJ11027. We amplified a partial sequence of the chiK using chitinase-specific primer sets. Using inverse PCR cloning method with two specific sets of PCR primers rather than single set, we identified the 4 kb DNA fragment containing chiK gene. From the fragment, we amplified the full chiK gene and cloned into pUC18 vector designated as pChiK. The recombinant E. coli clones harboring pChiK produced a clear zone around the colony in the colloidal chitin plates. Open reading frame of the chiK is 2553 bplong encoding a ChiK precursor protein of 851 amino acids, which consist of N-terminal signal peptide of 21 amino acids and a mature ChiK protein with 830 amino acids. The deduced amino acid sequences of chiK show 89% identity with that of Vibrio carchariae chitinase. The conserved module of Vibrio sp. 98CJ11027 ChiK indicates that the ChiK belongs to the family 18 of glycoside hydrolases.-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.titleMolecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027-
dc.title.alternative해양미생물 Vibrio sp. 98CJ11027에서 특이한 Chitinase 유전자의 분리-
dc.typeProceeding-
dc.identifier.bibliographicCitationLee, Yoo Kyung, Lee, Hong Kum, 이재형. 2004. Molecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027. 2004 International Meeting of the Microbiological Society of Korea. Choenan Sangnok Convention center. 2004.05.13~14.-
dc.citation.volume1-
dc.citation.number1-
dc.citation.conferenceDate2004.05.13~14-
dc.citation.conferenceName2004 International Meeting of the Microbiological Society of Korea-
dc.citation.conferencePlaceChoenan Sangnok Convention center-
dc.description.articleClassificationPro(초록)국내-
dc.subject.keywordVibrio sp. 98CJ11027-
dc.subject.keywordchitinase-
dc.subject.keywordglycoside hydrolase-
dc.subject.keywordinverse PCR cloning-
dc.subject.keywordmarine bacterium-
dc.identifier.localId2004-0051-
Appears in Collections  
2004-2005, 시계열 퇴적물트랩을 이용한 남극해 물질 순환연구 및 남극 미생물 활용 기술 개발 (04-05) / Shin, Hyoung Chul (PP04104, PE05002)
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