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Diversity of Marine Bacterial Communities in Arctic Biofilms using Cultivation and Cultivation-independent methods

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Title
Diversity of Marine Bacterial Communities in Arctic Biofilms using Cultivation and Cultivation-independent methods
Other Titles
북극 해양 생물막 세균의 다양성 분석
Authors
Cho, Hyun Hee
Cho, Kyeung Hee
Lee, Yoo Kyung
Lee, Hong Kum
Keywords
Bacterial diversity; Cytophaga-Flavobacterium group; Gram positive bacteria; marine biofilm; proteobacteria
Issue Date
2005
Citation
Cho, Hyun Hee, et al. 2005. Diversity of Marine Bacterial Communities in Arctic Biofilms using Cultivation and Cultivation-independent methods. 한국미생물연합회. 한국미생물연합회. 2005.10.14~.
Abstract
Bacterial diversity in Arctic marine biofilms was analyzed through cultivation and cultivation –
independent methods. We collected biofilms formed on a floating-pier and a plastic cable under seawater which were located around the Korea Arctic Research Station Dasan at Ny-Alesund, Svalbard, Norway (79ON, 12OE). A total of 95 strains were cultured, and 22 species were finally identified by phylogenetic analysis on the basis of 16S rDNA sequences. They belonged to γ-proteobacteria, Gram high GC+, Cytophaga-Flavobacterium group. The phylogenetic analysis of 110 clones which were randomly selected from 16S rDNA clone libraries, showed that about 54% of the clones belonged to Cytophaga-Flavobacterium group. The most frequent clone was an uncultured Cytophaga sp. DGGE of the partially amplified 16S rDNA was performed and DNA fragments in the most intense bands were sequenced. The majority of retrieved sequences were associated with an uncultured bacterium and an uncultured Cytophaga sp. Diversity of cultured bacteria was quite different from bacterial diversity obtained from cultivation-independent methods.
URI
http://repository.kopri.re.kr/handle/201206/7577
Conference Name
한국미생물연합회
Conference Place
한국미생물연합회
Conference Date
2005.10.14~
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