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Cloning and Expression of a Chitinase from an Antarctic Bacterium, Sanguibacter sp. KCTC10714

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dc.contributor.authorYim, Joung Han-
dc.contributor.authorHong, Soon Gyu-
dc.contributor.authorLee, Yoo Kyung-
dc.contributor.authorKim, Il-Chan-
dc.contributor.authorKim, In Hee-
dc.contributor.authorLee, Chang-Eun-
dc.contributor.authorLee, Hong Kum-
dc.coverage.spatialKing George Islanden_US
dc.coverage.spatialKing Sejong Station-
dc.date.accessioned2018-04-05T06:19:43Z-
dc.date.available2018-04-05T06:19:43Z-
dc.date.issued2006-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/7614-
dc.description.abstractWe isolated a chitinase-producing bacterium strainKCTC10714 from sea sand that had been collected around the King Sejong Station, King George Island in Antarctica (62° 13′ S, 58° 47′ W). It was identified as Sanguibacter sp., based on the biochemical properties and 16S rRNA gene sequence. KCTC10714 chitinase degraded chitin to produce chitinoligomer and chitobiose. KCTC10714 chitinase showed enzyme activity in broad range of pH 4 - 9, and temperature from 0 to 70℃. At 0℃, it showed 70.9% of relative activity in comparison with 100% at 45℃. We cloned the chitinase gene from KCTC10714 using inverse PCR cloning method. KCTC10714 chitinase gene was 1,888 bp in length including 388 bp of 5'-UTR and 51 bp of 3'-UTR, and it was designated as chi21702. The ORF of chi21702 consisted of 1,449 bp (482 amino acid), and contained ChtBD3 (a chitin/cellulose binding domain) and an active site for chitinase family 18. We also confirmed that the recombinant protein of chi21702 made approximately 56 kDa after overexpression in E.coli system. The characterization of the recombinant Chi21702 is under investigation.en_US
dc.languageEnglish-
dc.subject.classificationKing Sejong Stationen_US
dc.titleCloning and Expression of a Chitinase from an Antarctic Bacterium, Sanguibacter sp. KCTC10714-
dc.title.alternative남극 세균 Santuibacter sp. KCTC10714 키틴분해효소의 클로닝과 발현-
dc.typeProceedingen_US
dc.identifier.bibliographicCitationYim, Joung Han, et al. 2006. Cloning and Expression of a Chitinase from an Antarctic Bacterium, Sanguibacter sp. KCTC10714. 2006 International Meeting of the Microbiological Society of Korea. Exco, Daegu. 2006.05.03~04.-
dc.citation.volume1en_US
dc.citation.number1en_US
dc.citation.conferenceDate2006.05.03~04en_US
dc.citation.conferenceName2006 International Meeting of the Microbiological Society of Koreaen_US
dc.citation.conferencePlaceExco, Daeguen_US
dc.coverage.x62° 13′Sen_US
dc.coverage.y58° 47′Wen_US
dc.description.articleClassificationPro(초록)국외-
dc.subject.keywordAntarctic Bacteriumen_US
dc.subject.keywordSanguibacteren_US
dc.subject.keywordchitinaseen_US
dc.subject.keywordcloningen_US
dc.subject.keywordgeneen_US
dc.identifier.localId2006-0050-
Appears in Collections  
2006-2010, Procurement and utilization of polar genetic resources (06-10) / Lee, Hong Kum; Yim, Joung Han (PE06050, PE07050, PE08050, PE09050, PE10050)
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