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Characterization of protease-producing bacteria from the arctic ocean

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Title
Characterization of protease-producing bacteria from the arctic ocean
Other Titles
북극해로부터 분리한 단백질분해효소 생성세균의 특성연구
Authors
Cho, Kyeung Hee
Yim, Joung Han
Lee, Hong Kum
홍일정
김윤민
Hong, Soon Gyu
Keywords
arctic sea; protease
Issue Date
2006
Citation
Cho, Kyeung Hee, et al. 2006. Characterization of protease-producing bacteria from the arctic ocean. Korean Society for Microbiology and Biotechnology. Korean Society for Microbiology and Biotechnology. 2006.06.19~.
Abstract
Biodiversity of protease-secreting bacteria was investigated from Barentz sea, Cara sea and Russian Harbor area. Microorganisms producing clear zone around the colony on the Zobell agar containing skim milk were isolated. They were grouped based on the RFLP pattern of amplified 16S rDNA and selected isolates from each RFLP group were identified by phylogenetic analysis of partial sequence of 16S rDNA using Kimura's 2-parameter method and neighbor-joining algorithm. As a result, protease producing microorganisms were identified as Bacillus cereus, Bacillus hwajinpoensis, Bacillus indicus, Bacillus licheniformis, Bacillus pumilus, Bacillus simplex, Bacillus subtilis, Bacillus sp., Halobacillus sp., Paenibacillus amylolyticus, Paenibacillus barcinonensis, and Pseudomonas sp. Cultural characteristics of isolates and relative activity of proteases depending on the temperature were compared.
URI
http://repository.kopri.re.kr/handle/201206/7657
Conference Name
Korean Society for Microbiology and Biotechnology
Conference Place
Korean Society for Microbiology and Biotechnology
Conference Date
2006.06.19~
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