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cDNA cloning and expression of glutathione S-transferase (GST) gene from an Arctic copepod Calauns glacialis

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Title
cDNA cloning and expression of glutathione S-transferase (GST) gene from an Arctic copepod Calauns glacialis
Other Titles
북극 플랑크톤 Calanus glacialis에서 글루타치오 S-전이효소 유전자의 cDNA 클로닝과 발현
Authors
이창은
Lee, Yoo Kyung
Cho, Hyun Hee
Kim, Il-Chan
Lee, Jin sung
Hong, Soon Gyu
Keywords
Arctic copoped; Calanus glacialis; GST; Zooplankton
Issue Date
2007
Citation
이창은, et al. 2007. cDNA cloning and expression of glutathione S-transferase (GST) gene from an Arctic copepod Calauns glacialis. 한국미생물 생명공학회. 한국미생물 생명공학회. 2007.06.28~.
Abstract
Copepod species of the genus Calanus are dominant components of the zooplankton biomass in the Arctic Ocean. One of these species, Calanus glacialis, is considered to be native to the arctic shelves and plays an important role in marine food webs, both as consumers of primary production and as prey species for the larvae of commercial fish. We collected a Copepod species from seawater around the Korea Arctic Research Station Dasan at Ny-Alesund, Svalbard, Norway (79ON, 12OE). The Arctic copepod was identified as Calanus gracialis using phylogenetic analysis based on the LSU rDNA gene
it showed 100% identity with the sequence of the Antarctic Calanus gracialis LSU rDNA gene. Using 5’ and 3’ rapid amplification of cDNA ends (RACE) PCR techniques, we cloned the glutathione S-transferase gene from the Arctic copepod Calanus gracialis. The ORF of GST consisted of about 609 bp. We also constructed the recombinant GST plasmid with a 6xHis-Tag at the N-terminal of GST cDNA. Recombinant Calanus gracialis-GST was expressed in transformed Escherichia coli. This is the first report on GST of the Arctic copepod Calanus gracialis.
URI
http://repository.kopri.re.kr/handle/201206/7681
Conference Name
한국미생물 생명공학회
Conference Place
한국미생물 생명공학회
Conference Date
2007.06.28~
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