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Screening and Partial Purification of Antifreeze Proteins from Polar Microalgae

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Title
Screening and Partial Purification of Antifreeze Proteins from Polar Microalgae
Authors
Seo, Ki-Won
Kim, Hak Jun
Kang, Sung-Ho
Keywords
antifreeze proteins; ice recrystallization; ice-structuring proteins; thermal hysteresis
Issue Date
2007
Citation
Seo, Ki-Won, Kim, Hak Jun, Kang, Sung-Ho. 2007. Screening and Partial Purification of Antifreeze Proteins from Polar Microalgae. 조류학회. 조류학회. 2007.05.11~.
Abstract
Antifreeze proteins (AFPs) can protect organisms from freezing injury by adsorbing to ice and inhibiting its growth. We screened the antifreeze activity from polar Microalgae and isolated more than 10 microalgal strains showing the activity. The presence of antifreeze activity was observed by nanolitre osmometer. Among them, two microalgal strains, KOPRI ArM0014 and KOPRI AnM0046, were further investigated and their antifreeze proteins were partially purified. The supernatant of culture in log phase were harvested and filtered through Tangential flow filtration (TFF). Then cold finger method which uses ice seed to let antifreeze protein to bind to the surface of an ice, is used to selectively adsorb and extract Antifreeze proteins (AFPs) from culture solutions of two strains. Extracellular Antifreeze proteins(AFPs) were analyzed by SDS-PAGE. The extracted proteins showed antifreeze activity and were subject to further characterization.
URI
http://repository.kopri.re.kr/handle/201206/7707
Conference Name
조류학회
Conference Place
조류학회
Conference Date
2007.05.11~
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