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Temperature-induced Loss of Linear Catabolic Megaplasmid (pDK2) in Rhodococcus sp. Strain DK17

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Title
Temperature-induced Loss of Linear Catabolic Megaplasmid (pDK2) in Rhodococcus sp. Strain DK17
Other Titles
Rhodococcus sp. strain DK17 대사관련 거대플라스미드(pDK2)의 열처리에 의한 소실
Authors
Kim, Dockyu
Ki Young Choi
Kim, Eungbin
Keywords
Alkylbenzene; Catabolic megaplasmid; Rhodococcus; Temperature-induced
Issue Date
2008
Citation
Kim, Dockyu, Ki Young Choi, Kim, Eungbin. 2008. Temperature-induced Loss of Linear Catabolic Megaplasmid (pDK2) in Rhodococcus sp. Strain DK17. 한국미생물학회연합. 한국미생물학회연합. 2008.10.16~.
Abstract
The actinomycete Rhodococcus sp. strain DK17 is able to grow on various alkylbenzenes including benzene, toluene, ethylbenzene, and o-xylene (BTEX). DK17 possesses three linear megaplasmids (380-kb pDK1, 330-kb pDK2, and 750-kb pDK3). The versatile metabolic capacity of DK17 is encoded on the catabolic linear pDK2, which has been previously shown to be partially deleted or even lost when DK17 cells exposed to UV light. However, complete loss of pDK2 at a higher rate of 2.5% was observed to spontaneously occur from several DK17 cells which were heat-treated at 37℃ for 48.8 min (the doubling time of DK17 at optimal 30℃). Due to the absence of the functional catabolic pDK2, all the mutants were unable to grow on ethylbenzene selected as a representative of alkylbenzene substrates, but retained the ability to degrade phthalate. The loss of pDK2 might be attributed to an innate instability of linear megaplasmids, especially in replication during cell division, at a higher ambient temperature above the host’s optimal range. It should be further investigated to conclude whether this is a common phenomenon among linear megaplasmids or not.
URI
http://repository.kopri.re.kr/handle/201206/7838
Conference Name
한국미생물학회연합
Conference Place
한국미생물학회연합
Conference Date
2008.10.16~
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