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Bacterial community structure of an alpine cryoconite sample, as revealed by pyrosequencing analyses

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Title
Bacterial community structure of an alpine cryoconite sample, as revealed by pyrosequencing analyses
Other Titles
크라이오코나이트의 세균 군집 구조
Authors
오현명
Rosa Margesin
Hong, Soon Gyu
Lee, Hong Kum
Franz Schinner
고유석
Cho, Kyeung Hee
Cho, Jang-Cheon
Kim, Kyung Mo
Keywords
community; cryoconite; pysosequencing
Issue Date
2008
Citation
오현명, et al. 2008. Bacterial community structure of an alpine cryoconite sample, as revealed by pyrosequencing analyses. Committee for Polar and Alpine Microbiology. Committee for Polar and Alpine Microbiology. 2008.05.11~.
Abstract
Variable domains, V1 and V2, of bacterial 16S rRNA gene were amplified with universal primers 27F and 338R from environmental DNA of a glacier cryoconite sample collected in the Ö
tztaler Alps in Austria (Rettenbachferner, altitude 2760 m above sea level). PCR products were directly sequenced by pyrosequencing method and a total of 31,427 sequences were obtained by the forward primer. 1,467 OTUs were recovered by 97% similarity cutoff and the highest OTU diversity was recovered from Bacteroidetes (371), and followed by Betaproteobacteria (247), Alphaproteobacteria (142), Actinobacteria (117), Deltaproteobacteria (76), Gammaproteobacteria (59), Cyanobaceria (50), and several minor divisions.
URI
http://repository.kopri.re.kr/handle/201206/7858
Conference Name
Committee for Polar and Alpine Microbiology
Conference Place
Committee for Polar and Alpine Microbiology
Conference Date
2008.05.11~
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