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Expression of Recombinant Endochitinase of Antarctic Sanguibacter sp. KCTC 13143 in Pichia pastoris

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Title
Expression of Recombinant Endochitinase of Antarctic Sanguibacter sp. KCTC 13143 in Pichia pastoris
Other Titles
남극 상귀박터종 KCTC 13143의 재조합 엔토카이티네이즈의 이스트 피키아 파스토리스에서의 발현
Authors
Koh, Hye Yeon
Kim, Dockyu
Hong, Soon Gyu
Yim, Joung Han
Lee, Hong Kum
Kim, Il-Chan
Lee, Sung Gu
Keywords
Antarctic; Endochitinases; NAG; Pichia; Recombinant
Issue Date
2008
Citation
Koh, Hye Yeon, et al. 2008. Expression of Recombinant Endochitinase of Antarctic Sanguibacter sp. KCTC 13143 in Pichia pastoris. 극지연구소. 극지연구소. 2008.09.24~.
Abstract
Chitin composes the cell walls of some animals and microbes, including insects, crustaceans, and fungi. Chitinases break down glycosidic bonds in chitin. In combination of exochitinases, endochitinases are considered as important enzymes in biomedical industry for producing particularly N-acetylglucosamine (NAG) and others. Endochitinase chi21702 was isolated from Antarctic Sanguibacter sp. KCTC 13143 and well characterized in our lab previously. The gene for this enzyme was obtained from the genomic DNA and the sequence was determined successfully. The methylotrophic Yeast Pichia pastoris expression system was applied to develop the production process of the enzyme since this system is known to facilitate the purification of the recombinant enzymes secreted to the culture media. The Pichia system expressed the recombinant Antarctic endochitinase successfully and revealed enzymatic activity using colloidal chitin as a substrate. The expressed protein showed higher molecular weight than theoretical one due to maybe post-translational modification, presumably glycosylation. This presentation introduces unique characteristics of chitinases from Antarctic bacteria and suggests a potential for the development of biomedical applications.
URI
http://repository.kopri.re.kr/handle/201206/7871
Conference Name
극지연구소
Conference Place
극지연구소
Conference Date
2008.09.24~
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