KOPRI Repository

Expression of Recombinant Endochitinase of Antarctic Sanguibacter sp. KOPRI 21702 in Pichia pastoris

Metadata Downloads
Title
Expression of Recombinant Endochitinase of Antarctic Sanguibacter sp. KOPRI 21702 in Pichia pastoris
Other Titles
남극 상귀박터 종 KOPRI 21702 유래의 재조합 엔도카이티네이즈의 피키아 파스토리스에서의 발현
Authors
Lee, Sung Gu
Lee, Hong Kum
Koh, Hye Yeon
Kim, Il-Chan
Yim, Joung Han
Kim, Dockyu
Hong, Soon Gyu
나득채
Keywords
Antarctic; Endochitinases; NAG; Pichia; Recombinant
Issue Date
2008
Citation
Lee, Sung Gu, et al. 2008. Expression of Recombinant Endochitinase of Antarctic Sanguibacter sp. KOPRI 21702 in Pichia pastoris. International Federation for Cell Biology. International Federation for Cell Biology. 2008.10.10~.
Abstract
Chitin composes the cell walls of some animals and microbes, including insects, crustaceans, and fungi. Chitinases break down glycosidic bonds in chitin. In combination of exochitinases, endochitinases are considered as important enzymes in biomedical industry for producing particularly N-acetylglucosamine (NAG) and others. Endochitinase chi21702 was isolated from Antarctic Sanguibacter sp. KOPRI 21702 and well characterized in our lab previously. The gene for this enzyme was obtained from the genomic DNA and the sequence was determined successfully. The methylotrophic Yeast Pichia pastoris expression system was applied to develop the production process of the enzyme since this system is known to facilitate the purification of the recombinant enzymes secreted to the culture media. The Pichia system expressed the recombinant Antarctic endochitinase successfully and revealed enzymatic activity using colloidal chitin as a substrate. The expressed protein showed higher molecular weight than theoretical one due to maybe post-translational modification, presumably glycosylation. This presentation introduces unique characteristics of chitinases from Antarctic bacteria and suggests a potential for the development of biomedical applications.
URI
http://repository.kopri.re.kr/handle/201206/7872
Conference Name
International Federation for Cell Biology
Conference Place
International Federation for Cell Biology
Conference Date
2008.10.10~
Files in This Item
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse