Purification and Characterization of Endochitinase from Antarctic Bacterium Sanguibacter antarcticus KCTC 13143
- Purification and Characterization of Endochitinase from Antarctic Bacterium Sanguibacter antarcticus KCTC 13143
- Other Titles
- 남극 유래 세균인 Sanguibacter antarcticus KCTC 13143가 생산하는 endochitinase의 분리 및 특성연구
- Park, Ha Ju
Yim, Joung Han
- Sanguibacter; cold-adapted; endochitinase; psychrotrophic
- Issue Date
- Park, Ha Ju, et al. 2008. Purification and Characterization of Endochitinase from Antarctic Bacterium Sanguibacter antarcticus KCTC 13143. 극지연구소. 극지연구소. 2008.09.25~.
- A psychrotrophic bacterium Sanguibacter antarcticus KCTC 13143 was previously isolated from sea sand on King Sejong Station in Antarctica. In this study we purified and characterized a cold-adapted chitinase from this bacterium. The chitinase was produced in ZoBell medium containing 0.4% swollen chitin at 25℃, and purified by two-step using a hydrophobic interaction chromatography (phenyl-sepharose) and a gel filtration chromatography. The purified chitinase had specific activities of 23.28 and 12.57 U/mg toward the substrates pNP-(GlcNAc)2 and pNP-(GlcNAc)3, respectively, while had no activity for pNP-GlcNAc. This results indicated that the chitinase from KCTC 13143 have an endo-specific activity. Temperature and pH for the optimal chitinase activity were determined to be 37 ℃ and pH 7.6, respectively. Moreover, the chitinase activity at 0 ℃ remained approximately 40% lower than at the optimal temperature. This enzyme was stable at 10-37 ℃ and pH 4-10, respectively. Also it was stable in the presence of various metal ions and detergents. Thus, the above characteristics of this chitinase shows that it could be developed as a useful cold-active biocatalyst in commercial processes.
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