KOPRI Repository

Characterization and chemical modification of a GlcNAc/GalNAc specific lectin, Bryohealin, from the marine green alga Bryopsis plumosa

Metadata Downloads
Title
Characterization and chemical modification of a GlcNAc/GalNAc specific lectin, Bryohealin, from the marine green alga Bryopsis plumosa
Other Titles
해양 녹조식물 Bryopsis plumosa로 부터 정제한 lectin, Bryohealin의 당 결합 자리 특성 연구
Authors
Jung, Min Gui
Choi, Han-Gu
Kang, Sung-Ho
Kim, Gwang Hoon
이기평
Keywords
Bryohealin; Bryopsis plumosa; N-acetyl-D-galactosamine; N-acetyl-D-glucosamine; lectin
Issue Date
2009
Citation
Jung, Min Gui, et al. 2009. Characterization and chemical modification of a GlcNAc/GalNAc specific lectin, Bryohealin, from the marine green alga Bryopsis plumosa. 한국조류학회. 한국조류학회. 2009.06.05~.
Abstract
Bryohealin is lectin isolated from the marine green alga Bryopsis plumosa. It was reported to be involved in the wound-healing process of B. plumosa. The lectin molecule is composed of two identical subunits of 27 kDa each, cross-linked by disulfide bond and showed binding specificity to N-acetyl-D-glucosamine and N-acetyl-D-galactosamine. We demonstrated characterization of a carbohydrate binding site of Bryohealin by chromatography and chemical modification methods. Bryohealin had identical two or more binding sites and shared same binding site for two different carbohydrates (GalNAc and GlcNAc). During chemical modification studies, hemagglutinating activities of Bryohealin were not affected by modification of histidine, tryptophan, asparticacid, and glutamicacid. When 50% of arginine residues were modified with 1, 2-cyclohexanedione, activity of Bryohealin rapidly decreased. Protection was observed when the arginine modification was performed in the presence of inhibitory sugar (0.15 M GalNAc). The results suggest that the modified arginine is included in the carbohydrate binding site of Bryohealin. Tryptic digested peptides containing modified arginine residues were separated by HPLC.
URI
http://repository.kopri.re.kr/handle/201206/7898
Conference Name
한국조류학회
Conference Place
한국조류학회
Conference Date
2009.06.05~
Files in This Item
There are no files associated with this item.
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse