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Effects of environmental stresses on the pigment compositions and physiological properties of Antarctic microalga Pyramimonas gelidicola

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Title
Effects of environmental stresses on the pigment compositions and physiological properties of Antarctic microalga Pyramimonas gelidicola
Other Titles
남극미세조 Pyramimonas gelidicola의 환경 스트레스에 의한 색소구성과 생리적 변화연구
Authors
Lee, Soo Young
Kim, Young Nam
Kim, Hak Jun
Kang, Sung-Ho
Keywords
Pigment; Pyramimonas gelidicola
Issue Date
2009
Citation
Lee, Soo Young, et al. 2009. Effects of environmental stresses on the pigment compositions and physiological properties of Antarctic microalga Pyramimonas gelidicola. 한국 미생물학회연합. 한국 미생물학회연합. 2009.10.22~.
Abstract
Antarctic flagellate Pyramimonas gelidicola (KOPRI AnM0046) is a dominant green algal species found in Southern Ocean and ice–
covered lakes such as Ace Lake. This microalga, one of the most important primary producers in those cold ecosystems, undergoes freeze-thaw cycles, extreme fluctuations in irradiance, large variations in nutrient and salinity. To understand how this species acclimates to these environmental changes, we investigated changes of pigment compositions and light utilization efficiency after cold shock treatment and light stress. Light stress was given by shifting P. gelidicola cultured at 4℃, 90 µ
mol m-2s-1 to 15 µ
mol m-2s-1 at the same temperature. Cold shock treatment was done by transferring P. gelidicola cultured in f/2 media at 8℃, 15 µ
mol m-2s-1 to 4℃ and -1℃ at the same light intensity. After these treatments, cells were harvested and analyzed with HPLC and Fluorescence Induction and Relaxation (FIRe). The FIRe is a technique to measure maximum quantum efficiency of photochemistry. Shifting to low irradiance resulted in 64% growth rate reduction while it led to 10% increase in the maximum quantum yield of photosynthesis (Fv/Fm). Pigment analysis using HPLC also validated the improved quantum yield, showing the augmented accessory light-harvesting pigment. The ratio of zeaxanthin to violaxanthin was 2.641:0.517 (High irradiance: Low irradiance). On the other hand, the effect of abrupt cold stress on the pigment compositions was insignificant. However the maximum quantum yield of photosynthesis was higher in the lower temperature than higher temperature, suggesting that photosynthesis of this microalga is optimized in the cold temperature. In conclusion, these data show that photosynthesis of P. gelidicola is optimized at lower temperature and that like mesophilic microalgae pigment composition of P. gelidicola at different light intensity was changed to adjust photon utilization efficiencies. Since P. gelid
URI
http://repository.kopri.re.kr/handle/201206/7947
Conference Name
한국 미생물학회연합
Conference Place
한국 미생물학회연합
Conference Date
2009.10.22~
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