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The effect of temperature and pH on psychrophilic chitinase expression in Pichia pastoris X-33

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Title
The effect of temperature and pH on psychrophilic chitinase expression in Pichia pastoris X-33
Other Titles
재조합 효모에 의한 저온성 chitinase의 발현에 미치는 온도 및 pH의 영향
Authors
Han, Se Jong
Park, Heeyong
Yim, Joung Han
Koh, Hye Yeon
Lee, Sung Gu
Keywords
Antarctic; Pichia; chitinase; psychrophilic
Issue Date
2009
Citation
Han, Se Jong, et al. 2009. The effect of temperature and pH on psychrophilic chitinase expression in Pichia pastoris X-33. (사) 한국생물공학회. (사) 한국생물공학회. 2009.11.02~.
Abstract
To confirm the optimal cultivation conditions of Pichia pastoris X-33 containing the psychrophilic chitinase gene cloned from Antarctic microorganism, Sanguibacter antarcticus KOPRI 21702, the effect of temperature and pH on chitinase expression was investigated in a jar fermentor. Expression of target protein was performed by methanol addition and confirmed by an activity assay with p-nitrophenyl-N-acetyl-
-D-glucosaminide as a substrate. Cells were grown at various temperatures ranging from 20 to 30 
C and pHs from 5.5 to 6.5. The chitinase production was divided into two phases: batch and induction fed-batch. Ultimately, the optimal temperature and pH for the production of chitinase were found to be 20 
C, pH 6.0, respectively.
URI
http://repository.kopri.re.kr/handle/201206/7962
Conference Name
(사) 한국생물공학회
Conference Place
(사) 한국생물공학회
Conference Date
2009.11.02~
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