KOPRIa litus gen. nov., sp. nov., of the Family Oxalobacteraceae, Isolated from Antarctic Coastal Seawater

Abstract

A Gram-negative, non-motile, catalase- and oxidase- positive, strictly aerobic and short-rod-shaped bacterium that was designated strain KOPRI 25157T was isolated from coastal seawater sample in around King Sejong Station, in King George Island, Antarctica. The temperature and pH ranges for growth on R2A agar were 10-20℃, and 5.0-10.0, respectively. Phylogenetic analyses of the 16S rRNA gene sequence of strain KOPRI 25157 T have showed that it belongs to the family Oxalobacteraceae of the class Betaproteobacteria, but it formed a distinct clade from other recognised members of the family. Major ubiquinone was Q-8. Predominant cellular fatty acids were C10:0 (0.2%), C10:0 3OH (3.2%), C12:0 (2.9%), C16:1 ω7c/15 iso 2OH (56.4%), C16:0 (30.5%), C18:1 ω7c (3.7%), C18:0 (0.7%), and 11 methyl C18:1 ω7c (2.4%). On the basis of these data, it is proposed that strain KOPRI 25157T is the representative of a novel genus, named Kopria gen. nov. is proposed in the family Oxalobacteriaceae. The type strain for Kopria litus sp. nov. is KOPRI 25157T (=JCM 16673T =KCTC 23040 T).