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Analysis of Bacterial Community Change in Arctic Marine Biofilm

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Title
Analysis of Bacterial Community Change in Arctic Marine Biofilm
Other Titles
북극해양환경에서 형성된 생물막의 박테리아 군집 분석
Authors
Kim, Eun Hye
Lee, Yung Mi
Hong, Soon Gyu
Cho, Jang-Cheon
Lee, Hong Kum
Cho, Kyeung Hee
Keywords
Arctic; Bacterial Community; Biofilm
Issue Date
2010
Citation
Kim, Eun Hye, et al. 2010. Analysis of Bacterial Community Change in Arctic Marine Biofilm. 한국미생물학회연합. 한국미생물학회연합. 2010.10.14~.
Abstract
We analyzed bacterial community change in marine biofilm samples collected from Ny-Alesund, Svalbard, Norway for 15 days and tried to isolate the strains of the major OTUs, as a basic study of signal transduction from reconstruction of biofilm in the laboratory. Pyrosequencing approach was applied to analyze microbial community of biofilm. Analyses of 23,780 sequence reads showed that communities of biofilm were consisted of Actinobacteria (0.63%), Bacteroidetes (44.52%), Cyanobacteria (1.90%), Firmicutes (0.95%), Alphaproteobacteria (37.65%), Betaproteobacteria (0.71%), Gammaproteobacteria (8.38%), Proteobacteria_unclassfied (1.37%), WS3 (0.10%), and so on. Especially, Flavobacteriales (9.84%) in Bacteroidetes, Rhodobacterales (13.67%) in Alphaproteobacteria and Alteromonadales (0.70%) in Gammaproteobacteria were the major components in the marine biofilm. An OTU had 98.8% sequence similarity with Sulfitobacter pontiacus occupied upto 50% of the total community at day 10. 688 colonies isolated from biofilm samples were physiologically characterized to obtain strains belonging to major OUT. We isolated 22 strains through this physiologic information and identified 12 strains. Bacterial cultures of Rhodococcus (4 strains), Salinibacterium (3 strains), Cryobacterium (2 strains), Demequina (1 strain), Sanguibacter (1strains), and Microbacterium (1 strain) were obtained and none of the bacterial cultures showed high similarity with major components of the biofilm.
URI
http://repository.kopri.re.kr/handle/201206/8015
Conference Name
한국미생물학회연합
Conference Place
한국미생물학회연합
Conference Date
2010.10.14~
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