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Microbial community change of the biofilm formed in the marine environment of Antarctica

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Title
Microbial community change of the biofilm formed in the marine environment of Antarctica
Other Titles
남극의 해앵에서 형성된 바이오필름에서 미생물 커뮤니티 변화
Authors
Lee, Yung Mi
Hong, Soon Gyu
Cho, Kyeung Hee
Lee, Hong Kum
Chun, Jongsik
Kim, Eun Hye
Keywords
GS-FLX sequencing; Marine biofilm; Microbial community
Issue Date
2010
Citation
Lee, Yung Mi, et al. 2010. Microbial community change of the biofilm formed in the marine environment of Antarctica. SCAR. SCAR. 2010.08.04~.
Abstract
The microbial community change of biofilms formed on the artificial surfaces in the marine environment of Antarctica for seven days was investigated. 16S rRNA gene amplicons were directly sequenced using GS-FLX sequencing machine. Approximately 4,000 to 5,000 sequence reads were obtained from each sample. They were clustered into 262 to 938 phylotypes by 97% similarity cutoff. Most of phylotypes were affiliated to Bacteroidetes and Gammaproteobacteria. The minor phyla were included Alphaproteobacteria, Firmicutes, Cyanobacteria, Actinobacteria, GN02, and OD1. The relative abundance of phylotypes assigned to Proteobacteria changed as follows for 7 days
38.0% - 59.4% - 66.5% - 80.6% -57.6% - 28.0% - 43.1%. Phylotypes assigned to Bacteriodetes were the major at the initial stage, decreased down to 12.7% at the fourth day and increased in the later stage. The most predominant phylotype comprising 65.3% on the fourth day was closely related to Pseudoalteromonas prydzensis (98.4% similarity).
URI
http://repository.kopri.re.kr/handle/201206/8039
Conference Name
SCAR
Conference Place
SCAR
Conference Date
2010.08.04~
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