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Temperature-dependenet transcriptome analysis of the arctic Chlamydomonas sp.

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Title
Temperature-dependenet transcriptome analysis of the arctic Chlamydomonas sp.
Other Titles
북극 클라미도모나스 종의 온도별 전사체 분석
Authors
Lee, Jong Eun
Park, Hyun
Cho, Sung-Mi
Kim, Sanghee
Keywords
Arctic; Chlamydomonas; Transcriptome; cold
Issue Date
2016
Citation
Lee, Jong Eun, et al. 2016. Temperature-dependenet transcriptome analysis of the arctic Chlamydomonas sp.. 11th International Congress on Extremophiles, 2016. 교토. 2016.09.12~2016.09.16.
Abstract
Chlamydomonas sp. is widely present in the Earth’s aquatic ecosystem and is worth as a model species based on environmental factors related to environmental stresses. As a part of a study on the environmental adaptation mechanisms that exist in polar Chlamydomonas, we selected an Arctic Chlamydomonas sp. strain from the KOPRI culture collection of polar microorganisms (KCCPM, KNF0024), which had been isolated from ice-covered freshwater from the Arctic. This strain shows psychrophilic growth. To explore its adaptation strategy in freezing environments and identify the genes related cold acclimation, we investigated its temperature-dependent transcriptome changes. Uni-gene sets of this strain were established through de-novo assembly. The assembled contigs were annotated and functionally classified according to the biological functions. We examined the candidate target genes whose expression differs in accordance with the temperature, clustered them by expression patterns. Finally, we compared its temperature-dependent transcriptome response with those of Chlamydomonas reinhardtii, a mesophilic species. Taken together, this dataset would be a valuable resource to understand the molecular mechanism behind Chlamydomonas’ adaptation under extreme and changing environment.
URI
http://repository.kopri.re.kr/handle/201206/8117
Conference Name
11th International Congress on Extremophiles, 2016
Conference Place
교토
Conference Date
2016.09.12~2016.09.16
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