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Lignocellulose-degrading Paenibacillus Isolates from Alaska Grassland

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Title
Lignocellulose-degrading Paenibacillus Isolates from Alaska Grassland
Other Titles
알래스카 툰드라 토양에서 분리한 Lignocellulose 분해세균 Paenibacillus sp.
Authors
Kang, Sia
Choi, Eunjoo
Ahn, Eunsol
Kim, Eungbin
Kim, Dockyu
Keywords
Lignocellulose; bacteria; lignolytic enzymes; subarctic; xylanase
Issue Date
2012
Citation
Kang, Sia, et al. 2012. Lignocellulose-degrading Paenibacillus Isolates from Alaska Grassland. 한국미생물학회. 한국미생물학회. 2012.05.03~.
Abstract
Lignocellulose, consisting of lignin, hemicelluloses, and cellulose, is the major structural component of woody plants and non-woody plants such as grass. These chemical properties of the components make lignocellulose an attractive substrate of biotechnological value. As an effort to unearth new sources of cold-active lignolytic enzymes, we have screened Alaska grassland soils for the presence of bacteria with the activity of xylanase. Xylanases randomly cleave the ß 1,4 backbone of xylan, which constitutes the major component of hemicelluloses. Xylanase activity was initially measured as the size of the clear zones around colonies and, based on the size, a total of 100 bacterial colonies were selected. The 16S rDNA sequencing using the primers 27F and 1492R showed that 90 out of the 100 isolates belong to the genus Paenibacillus with various levels of 16S rDNA identity. Ten of the Paenibacillus isolates were found to grow well on different monomeric and dimeric components of lignocelluloses including vanilic acid, cinnamic acid, coumaric acid, and cellulobiose. Also, it is noteworthy that eight of the ten Paenibacillus isolates exhibit significant caseinase enzyme activity.strate of biotechnological value. As an effort to unearth new sources of cold-active lignolytic enzymes, we have screened Alaska grassland soils for the presence of bacteria with the activity of xylanase. Xylanases randomly cleave the ß 1,4 backbone of xylan, which constitutes the major component of hemicelluloses. Xylanase activity was initially measured as the size of the clear zones around colonies and, based on the size, a total of 100 bacterial colonies were selected. The 16S rDNA sequencing using the primers 27F and 1492R showed that 90 out of the 100 isolates belong to the genus Paenibacillus with various levels of 16S rDNA identity. Ten of the Paenibacillus isolates were found to grow well on different monomeric and dimeric components of lignocelluloses including vanilic acid, cinn
URI
http://repository.kopri.re.kr/handle/201206/8432
Conference Name
한국미생물학회
Conference Place
한국미생물학회
Conference Date
2012.05.03~
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