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Characterization of the cold-adapted α-tubulin from the psychrophilic polar mircroalgae

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Title
Characterization of the cold-adapted α-tubulin from the psychrophilic polar mircroalgae
Other Titles
극지미세조류들에서 분리해낸 tubulin 염기서열과 단백질서열의 비교 분석
Authors
Jung, Min Gui
Han, Jong Won
Choi, Han-Gu
Kang, Sung-Ho
Kim, Gwang Hoon
Kim, Sanghee
Kim, Min-Jung
Keywords
microtubules; polymerization; psychrophilic microalgae
Issue Date
2011
Citation
Jung, Min Gui, et al. 2011. Characterization of the cold-adapted α-tubulin from the psychrophilic polar mircroalgae. IASC,. IASC,. 2011.12.07~.
Abstract
Although microtubules usually disassemble at low temperature below 4℃ by disturbing polymerization of tubulin dimer, organisms living in a cold environment seem to overcome this limit by amino acid substitution of tubulin. We aimed to investigate whether amino acid substitution on tubulin occur commonly in polar microalgae and is responsible for promoting growth at freezing temperature at which non-cold adapted algae seldom grow and eventually die. The full-length cDNAs of α-tubulin from eight genera with 14 microalgal strains (ArF04, ArF08, ArF13, ArF24, ArF25, ArF26, ArF27, ArF28, ArF29, ArF32, AnF48, AnM08, AnM30, and AnM45) collected from the Arctic and the Antarctic were obtained by RACE and compared them with that of α-tubulin from mesophilic alga, Chlamydomonas reinhardtii. We found that the several amino acid substitutions occurred in most tubulins of polar microalgae. These substitutions were found in overlapped sites reported in previous work, presumably due to selective pressure of cold environment. Of the substituted sequences, the A295V region was conspicuous, which has reported that play an important role in the producing protofilament due to the increasing hydrophobicity. 10 out of 14 microalgal strains (72%) showed a substitution of alanine (A) to valine (V) in the 295th residue demonstrating that the substitution of A295V region is a largely conserved feature among polar 5544microorganisms
URI
http://repository.kopri.re.kr/handle/201206/8463
Conference Name
IASC,
Conference Place
IASC,
Conference Date
2011.12.07~
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