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Rapid discovery of antifreeze proteins from arctic microalga, ArF0006 (Chlamydomonas sp.) using 454 pyrosequencing

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Title
Rapid discovery of antifreeze proteins from arctic microalga, ArF0006 (Chlamydomonas sp.) using 454 pyrosequencing
Other Titles
북극미세조류 ArF0006으로 부터 분리해낸 결빙방지단백질들
Authors
Kim, Sanghee
Choi, Han-Gu
Jung, Min Gui
Kang, Sung-Ho
Lee, Soo Young
Kim, Min-Jung
Keywords
454 pyrosequencing; ArF0006; antifreeze protein; arctic micrlalgae
Issue Date
2011
Citation
Kim, Sanghee, et al. 2011. Rapid discovery of antifreeze proteins from arctic microalga, ArF0006 (Chlamydomonas sp.) using 454 pyrosequencing. IASC,. IASC,. 2011.12.06~.
Abstract
As part of an effort to discover antifreeze proteins (AFPs) in polar organisms, we have performed massively parallel pyroseqeuncing from a cDNA library of ArF0006, the arctic Chlamydomonas sp. grown at 4℃. AFP directly bind to the ice surface, inhibit the growth of ice crystals and reduce the possible damage within frozen tissues. These proteins have been described only in species inhabiting cold environments. Therefore, to investigate molecular adaptation such as AFPs, ArF0006 is an ideal candidate because it is collected in spots near the Dasan Station located NyÅlesund, Spitsbergen, Norway (78° 55N, 11° 56E), considered to be an arctic endemic species based on rbcL gene sequence data and whole genome sequence of its closest genera, chlamydomonas reinhardtii are already available for comparison. A total of 64,710 reads were generated, which assembled into 2,377 contigs and 19,424 singletons. Also 21,801 unique sequences were annotated with GO terms. The length of contigs ranges from 100 bp to 4,673 bp with an average of 665 bp, and that of singletons ranges from 50 bp to 1,114 bp with an average of 331 bp. The comparative analysis with ESTs of Chlamydomonas reinhardtii, Ostreococcus tauri, or Arabidopsis thaliana indicated that a large amount of ESTs derived from ArF0006 have not yet been detected or did not show similarity to publicly available any other genes. In th
URI
http://repository.kopri.re.kr/handle/201206/8467
Conference Name
IASC,
Conference Place
IASC,
Conference Date
2011.12.06~
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