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Structural basis for the Antifreeze activity of an Ice Binding Protein (LeIBP) from Arctic yeast

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Title
Structural basis for the Antifreeze activity of an Ice Binding Protein (LeIBP) from Arctic yeast
Other Titles
북극효모유래 결빙방지단백질의 고해상도 삼차 구조연구
Authors
Park, Kyoung Sun
Kim, Eunjung
Lee, Jun Hyuck
Kang, Sung-Ho
Kim, Hak Jun
Lee, Sung Gu
Park, Jong-chan
Do, Hackwon
Keywords
Antifreeze protein; Arctic yeast; Crystal structure; Leucosporidium sp.; ice binding protein
Issue Date
2011
Citation
Park, Kyoung Sun, et al. 2011. Structural basis for the Antifreeze activity of an Ice Binding Protein (LeIBP) from Arctic yeast. 한국생물공학회. 한국생물공학회. 2011.12.12~.
Abstract
Psychrophilic Arctic yeast, Leucosporidium sp. produces a glyco-ice binding protein (LeIBP) with a molecular mass of about 25 kDa that has a unique ability to lower the freezing point below the melting point once it binds to ice. Antifreeze proteins (AFPs) and ice binding proteins (IBPs) are found in many polar organisms and inhibit the growth of ice crystals to protect themselves from freezing. LeIBP exhibits the least protein sequence similarity with other previous known antifreeze protein structures. To better understand the ice binding mechanism of LeIBP, we have expressed full-length recombinant LeIBP in large amounts in Escherichia coli and purified the proteins. Here, we report the crystal structure of LeIBP at 1.57 Å resolution. Structural analysis of LeIBP revealed a dimeric right-handed β-helix fold, which is composed of three parts: a large coiled structural domain, a long helix region (Residues 96–115 form a long α-helix which packs along one face of the β-helix) and a C-terminal hydrophobic loop region (243-PFVPAPEVV-251). Unexpectedly, the C-terminal hydrophobic loop region has an extended conformation pointing away from the body of the coiled structural domain and forms an intertwined dimer interactions. From this structure we propose an ice binding sites, which is flat and contains a regular pattern of aligned Thr, Ala and Ser residues. Furthermore, we su
URI
http://repository.kopri.re.kr/handle/201206/8471
Conference Name
한국생물공학회
Conference Place
한국생물공학회
Conference Date
2011.12.12~
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