Isolation and characterization of Antarctic bacteria with protease activities
- Isolation and characterization of Antarctic bacteria with protease activities
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- 단백질분해효소 활성을 나타내는 남극 토양 미생물의 분리 및 특성 연구
- Yoo, Miyoun
Sohn, Min Ji
Oh, Yu Jin
- Antarctic; Protease; bacteria; screening
- Issue Date
- Yoo, Miyoun, et al. 2011. Isolation and characterization of Antarctic bacteria with protease activities. 한국미생물학회연합회. 한국미생물학회연합회. 2011.10.14~.
- Proteases, being one of the largest groups of industrial enzymes, have been used in a
wide range of applications including protein stain removal, fuel, food preparation,
biofilm removal, leather preparation, and so on. Microorganisms represent an excellent
source of protease enzymes owing to their broad biochemical diversity and their
susceptibility to genetic manipulation. As an effort to unearth new microbial resources
for proteases, we have screened Antarctic soils for the presence of proteolytic bacteria
using casein and keratin as substrates, respectively. A total of 169 bacterial genera
were identified including Pseudomonas (109), Bacillus (9), Thermoleophilum (11), Micro-
coccus (6), Chryseobacterium (2), Arthrobacter (3), Stenotrophomonas (3), Sporocarcina
(2), Paenibacillus (1), Planococcus (1), and unclassified Bacteria (22) after screening
100 different soil samples. Strains showing keratinase activity were further screened
for caseinase enzyme activity. 28 strains from 53 strains showing keratinase enzyme
activity also showed caseinase enzyme activity.
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