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Screening and Identification of Chitinase Genes from Arctic Marine Bacterium Pseudoalteromonas issachenkonii KOPRI 22718

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Title
Screening and Identification of Chitinase Genes from Arctic Marine Bacterium Pseudoalteromonas issachenkonii KOPRI 22718
Other Titles
북극해양 미생물 Pseudoalteromonas issachenkonii KOPRI 22718로부터 chitinase 유전자 탐색
Authors
Kim, Sung Jin
Kim, Dockyu
Yim, Joung Han
Kim, Tai Kyoung
Park, Ha Ju
Keywords
Arctic sea; Pseudoalteromonas; exo-acting chitinase; psychrotolerant
Issue Date
2011
Citation
Kim, Sung Jin, et al. 2011. Screening and Identification of Chitinase Genes from Arctic Marine Bacterium Pseudoalteromonas issachenkonii KOPRI 22718. 한국미생물학회. 한국미생물학회. 2011.05.12~.
Abstract
The psychrotolerant Pseudoalteromonas issachenkonii KOPRI 22718 was isolated for high exo-acting chitinase activity in Arctic sea. Previously, an exo-acting chitinase was purified from the culture supernatant and characterized on its biochemical reactions. To identify exochitinase encoding genes, genomic and small-insert DNA library were constructed by using pCC1FOS fosmid/E. coli EPI300 and high-copy plasmid pUC19/ E. coli Rosetta systems, respectively. The transformants from each library were screened on LB/colloidal chitin (0.55%), and finally one superior pUC19 clone was selected for the ability to form clear-zone around the cells. A cluster of three closely linked chitinase genes were revealed as organized in the order of chi III, chi II and chi I on the ~7.5 kb insert DNA. Based on functional analysis for conserved domains, it was predicted that chi III and chi II possess only one catalytic domain and one chitin binding domain, respectively, while chi I has one catalytic domain and one chitin binding domain. Now, chi III is being studied on its over-expression in E. coli and exo-chitinolytic function. This work was supported by a grant to KOPRI (PE11060).
URI
http://repository.kopri.re.kr/handle/201206/8504
Conference Name
한국미생물학회
Conference Place
한국미생물학회
Conference Date
2011.05.12~
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