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Screening and characterization for cold-active protease-producing bacteria from Bikal Lake

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Title
Screening and characterization for cold-active protease-producing bacteria from Bikal Lake
Other Titles
Bikal 호수로부터 저온활성 단백질분해효소 생산균주의 스크리닝 및 특성연구
Authors
Kang, Pilsung
Lee, Yung Mi
Kim, Dockyu
Park, Ha Ju
Yim, Joung Han
Park, Heeyong
Keywords
Bikal; bacteria; cold-active; protease; screening
Issue Date
2011
Citation
Kang, Pilsung, et al. 2011. Screening and characterization for cold-active protease-producing bacteria from Bikal Lake. 한국미생물학회. 한국미생물학회. 2011.05.12~.
Abstract
The Korea Polar Research Institute (KOPRI) had collected heterotrophic bacterial strains from water and ice of Baikal Lake. To identify excellent protease-producers among the bacterial collection (50 strains), 43 strains were examined for their extracellular protease activity on R2A plates supplemented with 1% skim milk at various temperatures (4-20 C). This rapid and direct screening method permitted the selection of a small group of 3 cold-adapted bacterial strains that showed proteolytic activities even at low temperature of 4oC. The cold-active proteases from these strains exhibited relative activities of 32%, 19%, and 15% at 5 C, respectively, compared to 100% activity at an optimal temperature of 40oC, and the molecular weights of the proteases were all estimated to be 75,000 Da by zymography. The proteases were classified into four categories (serine protease, aspartic protease, cysteine protease, and metalloprotease) according to the extent of enzymatic inhibition by a class-specific protease inhibitor. Since highly active and/or cold-adapted proteases have a potential for industrial or commercial enzymes, the protease-producing bacteria selected in this work will be studied as a valuable natural source for new proteases. This work was supported by a grant to KOPRI (PE11060).
URI
http://repository.kopri.re.kr/handle/201206/8505
Conference Name
한국미생물학회
Conference Place
한국미생물학회
Conference Date
2011.05.12~
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