KOPRI Repository

Biphenyl hydroxylation enhanced by an engineered o-xylene dioxygenase from Rhodococcus sp. strain DK17

Cited 0 time in wos
Cited 0 time in scopus

Full metadata record

DC Field Value Language
dc.contributor.authorKim, Eungbin-
dc.contributor.authorKang, Beom Sik-
dc.contributor.authorKim, Dockyu-
dc.contributor.authorYoo, Miyoun-
dc.date.issued2011-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/8506-
dc.description.abstractHydroxylation of the nongrowth substrate biphenyl by recombinant o-xylene dioxygenases from Rhodococcus sp. strain DK17 was studied through bioconversion experiments. The metabolites from the biphenyl hydroxylation by each enzyme were identified and quantified by gas chromatography-mass spectrometry. The L266F mutant enzyme produced much more 2 hydroxybiphenyl (2.43 vs. 0.1 μg/L) and 3 hydroxybiphenyl (1.97 vs. 0.03 μ g/L) than the wild-type. Site-directed mutagenesis combined with structural and functional analyses indicated that hydrophobic interactions and shielding effects against water are important factors in the hydroxylation of biphenyl by the o xylene dioxygenase. The residue at position 266 plays a key role in coordinating the reaction.-
dc.languageEnglish-
dc.titleBiphenyl hydroxylation enhanced by an engineered o-xylene dioxygenase from Rhodococcus sp. strain DK17-
dc.title.alternativeRhodococcus sp. strain DK17 유래 o-xylene dioxygenase에 의한 biphenyl hydroxylation 반응-
dc.typeProceeding-
dc.identifier.bibliographicCitationKim, Eungbin, et al. 2011. Biphenyl hydroxylation enhanced by an engineered o-xylene dioxygenase from Rhodococcus sp. strain DK17. 한국미생물학회. 한국미생물학회. 2011.05.12~.-
dc.citation.volume1-
dc.citation.number1-
dc.citation.conferenceDate2011.05.12~-
dc.citation.conferenceName한국미생물학회-
dc.citation.conferencePlace한국미생물학회-
dc.description.articleClassificationPro(초록)국내-
dc.subject.keywordRhodococcus-
dc.subject.keywordbiphenyl-
dc.subject.keywordhydoxylation-
dc.subject.keywordmutant-
dc.subject.keywordo-xylene dioxygenase-
dc.identifier.localId2011-0265-
Appears in Collections  
2011-2013, Utilization of novel metabolites from polar organisms (11-13) / Yim, Joung Han (PE11060, PE12040, PE13040)
Files in This Item
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse