KOPRI Repository

북극 척치해 심층수의 원생미생물 다양성

Metadata Downloads
Title
북극 척치해 심층수의 원생미생물 다양성
Authors
Kim, Ok-Sun
Lee, Bang Yong
Han, Duk Ki
Lee, Yoo Kyung
Kim, Hye Min
Nam, Sungjin
Keywords
Arctic Ocean; Chuchi Sea; deep-seawater; microbial diversity; pyrosequencing
Issue Date
2011
Citation
Kim, Ok-Sun, et al. 2011. 북극 척치해 심층수의 원생미생물 다양성. IASC, KOPRI ect.. IASC, KOPRI ect.. 2011.03.28~.
Abstract
The microbial diversity of deep-seawater from Chuchi Sea in Arctic Ocean was analyzed by using pyrosequencing method. Marine samples were collected from the first expedition of ice breaker ARAON from a cold deep-sea (depth: 2,400 m; temp.: - 0.375℃) in August 2010. The total 8,774 bacterial reads and 3,335 Archaeal reads were obtained from the pyrosequencing analysis, and all sequences were assigned in 97% similarity level. Among the bacteria, Proteobacteria was the most dominant phylum (89.8%), and followed by Bacteroidetes (7.9%). The most frequent bacterial sequence was assigned as Pelagibacter ubique (30.1%) belonged to the order SAR11of the class Alpha-proteobacteria. The SAR 11, of which Pelagibacter ubique is a representative, are found throughout the world’s oceans and dominant aerobic heterotrophs in marine surface waters. Pelagibacter ubique plays a key role in the oxidation of the oceanic dissolved organic carbon pool. In Archaea, Thaumarchaeota was the most dominant phylum (92.2%), and followed by Euryarchaeota (6.9%). In the case of Archaea, almost sequences of the Thaumarchaeota were assigned as an order of Cenarchaeales (91.3%), which consists of genera AY316120 (79.3%) and Nitrosopumilus (11.6%). The major sequences of AY316120 were described as “uncultured crenarchaeote DeepAnt EC39”. The DeepAnt EC39 appears to represent a lineage specific to deep-sea waters. These sequences are closely related to other mesophilic or psychrophilic crenarchaeotes. The aim of this study is to understand the prokaryotic microbial diversity in Arctic ocean, and the marine microbial diversity can be readily monitored by pyrosequencing of 16S rRNA gene.
URI
http://repository.kopri.re.kr/handle/201206/8516
Conference Name
IASC, KOPRI ect.
Conference Place
IASC, KOPRI ect.
Conference Date
2011.03.28~
Files in This Item
There are no files associated with this item.
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse