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Roles of Hydrophobic Interactions in the Hydroxylation of Biphenyl by o-Xylene Dioxygenase from Rhodococcus sp. Strain DK17

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Title
Roles of Hydrophobic Interactions in the Hydroxylation of Biphenyl by o-Xylene Dioxygenase from Rhodococcus sp. Strain DK17
Other Titles
Rhodococcus sp. strain DK17 유래 o-xylene dioxygenase의 바이페닐 산소화반응에 있어 소수성반응의 역할
Authors
Kim, Dockyu
Yoo, Miyoun
Kang, Beom Sik
Kim, Eungbin
Keywords
Biphenyl; Hydrophobic Interaction; Rhodococcus; o Xylene dioxygenase
Issue Date
2010
Citation
Kim, Dockyu, et al. 2010. Roles of Hydrophobic Interactions in the Hydroxylation of Biphenyl by o-Xylene Dioxygenase from Rhodococcus sp. Strain DK17. 한국미생물학회연합. 한국미생물학회연합. 2010.10.14~.
Abstract
Bioconversion experiments were performed against the non-growth substrates biphenyl using recombinant o-xylene dioxygenases from Rhodococcus sp. strain DK17. Hydroxylation metabolites of biphenyl by each enzyme were identified and quantified by a gas chromatography-mass spectrometry. The mutant enzyme with the substitution of L266 to phenylalanine produced approximately 24 and 66 times as much 2 hydroxybiphenyl and 3-hydroxybiphenyl as the wild type, respectively. Further site-directed mutagenesis combined with structural and functional analyses indicates that hydrophobic interactions and shielding effects against water as important factors in the hydroxylation of biphenyl by the DK17 o xylene dioxygenase and that the residue at the position 266 plays a key role in such coordination.
URI
http://repository.kopri.re.kr/handle/201206/8536
Conference Name
한국미생물학회연합
Conference Place
한국미생물학회연합
Conference Date
2010.10.14~
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