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Transcriptome analysis of Pseudomonas sp. from subarctic tundra soil: pathway description and gene discovery for humic acids degradation

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Title
Transcriptome analysis of Pseudomonas sp. from subarctic tundra soil: pathway description and gene discovery for humic acids degradation
Other Titles
아북극 툰드라 토양 Pseudomonas sp.의 전사체 분석: 부식질 분해 유전자와 분해경로 탐색
Authors
Kim, Dockyu
Park, Hyun
Sul, Woo Jun
Park, Ha Ju
Subject
Biotechnology & Applied Microbiology; Microbiology
Keywords
biodegradation; degradation pathway; humic substances; low temperature; soil bacteria
Issue Date
2017-12-01
Citation
Kim, Dockyu, et al. 2017-12-01. "Transcriptome analysis of Pseudomonas sp. from subarctic tundra soil: pathway description and gene discovery for humic acids degradation". FOLIA MICROBIOLOGICA, 63(3): 315-323.
Abstract
Although humic acids (HA) are involved in many biological processes in soils and thus their ecological importance has received much attention, the degradative pathways and corresponding catalytic genes underlying the HA degradation by bacteria remain unclear. To unveil those uncertainties, we analyzed transcriptomes extracted from Pseudomonas sp. PAMC 26793 cells time-dependently induced in the presence of HA in a lab flask. Out of 6,288 genes, 299 (microarray) and 585 (RNA-seq) were up-regulated by >2.0-fold in HA-induced cells, compared with controls. A significant portion (9.7% in microarray and 24.1% in RNA-seq) of these genes are predicted to function in the transport and metabolism of small molecule compounds, which could result from microbial HA degradation. To further identify lignin (a surrogate for HA) degradative genes, 6,288 protein sequences were analyzed against CAZy database and a self-curated list of putative lignin degradative genes. Out of 19 genes predicted to function in lignin degradation, several genes encoding laccase, dye-decolorizing peroxidase, vanillate O-demethylase oxygenase and reductase, and biphenyl 2,3-dioxygenase were up-regulated >2.0-fold in RNA-seq. This induction was further confirmed by qRT-PCR, validating the likely involvement of these genes in the degradation of HA.
URI
http://repository.kopri.re.kr/handle/201206/9429
URI
http://pamc.kopri.re.kr/strain/PAMC%2026793
DOI
http://dx.doi.org/10.1007/s12223-017-0573-0
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