https://repository.kopri.re.kr/handle/201206/11541 2026-04-05T23:05:46Z 2026-04-05T23:05:46Z Jo, Dong Gyu https://repository.kopri.re.kr/handle/201206/13142 2022-03-24T07:15:35Z 2021-07-20T00:00:00Z

Title: Molecular mechanism of Ramalin and Ramalin derivatives-mediated anti-inflammatory effects Authors: Jo, Dong Gyu

2021-07-20T00:00:00Z Kim, Jung Eun Min, Seul Ki Hong, Ju-Mi Kim, Kyung Hee Han, Se Jong Yim, Joung Han Park, Hyun Kim, Il-Chan https://repository.kopri.re.kr/handle/201206/11843 2022-03-24T07:14:40Z 2020-12-01T00:00:00Z

Title: Anti-inflammatory effects of methanol extracts from the Antarctic lichen, Amandinea sp. in LPS-stimulated raw 264.7 macrophages and zebrafish Authors: Kim, Jung Eun; Min, Seul Ki; Hong, Ju-Mi; Kim, Kyung Hee; Han, Se Jong; Yim, Joung Han; Park, Hyun; Kim, Il-Chan Abstract: The aim of the present study was to determine the anti-inflammatory effect of an extracts isolated from the lichen. Amandinea sp. was collected from the Antarctic and extracted with methanol. The basic screening of the anti-inflammatory property of the extracts was done using the NO assay. The extracts showed very little cytotoxicity, and reduced NO production in LPS-stimulated RAW 264.7 cells in a dose-dependent manner. Furthermore, the extracts inhibited LPS-induced release of pro-inflammatory cytokines such as interleukin-6 (IL-6), and tumor necrosis factor-α(TNF-α), and inflammatory mediators inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). The extracts also reduced the cytosolic p-IκB-α level and the level of the nuclear factor p65. We examined the anti-inflammatory effects of the extracts using zebrafish in vivo. The extracts reduced the amount of reactive oxygen species (ROS) in LPS-induced zebrafish larvae and inhibited the mRNA expression of inflammatory cytokines and mediators in a tail-cutting induced model. These results are similar to those obtained in vitro with RAW 264.7 cells. Collectively, the data suggest that the extracts may contain one of more compounds with anti-inflammatory effects. Further studies are required to identify the candidate compound/s and to understand the mechanism of action of the extract.

2020-12-01T00:00:00Z Park, Jun Chul Kim, Duck-Hyun Lee, Yoseop Lee, Min-Chul Kim, Tai Kyoung Yim, Joung Han Lee, Jae-Seong https://repository.kopri.re.kr/handle/201206/11796 2022-03-24T07:14:09Z 2020-12-01T00:00:00Z

Title: Genome-wide identification and structural analysis of heat shock protein gene families in the marine rotifer Brachionus spp.: Potential application in molecular ecotoxicology Authors: Park, Jun Chul; Kim, Duck-Hyun; Lee, Yoseop; Lee, Min-Chul; Kim, Tai Kyoung; Yim, Joung Han; Lee, Jae-Seong Abstract: Heat shock proteins (Hsp) are class of conserved and ubiquitous stress proteins present in all living organisms from primitive to higher level. Various studies have demonstrated multiple cellular functions of Hsp in living organisms as an important biomarker in response to abiotic and biotic stressors including temperature, salinity, pH, hypoxia, environmental pollutants, and pathogens. However, full understanding on the mechanism and pathway involved in the induction of Hsp still remains challenging, especially in aquatic invertebrates. In this study, the entire Hsp family and subfamily members in the marine rotifers Brachionus spp., one of the cosmopolitan ecotoxicological model organisms, have been genome-widely identified. In Brachionus spp. Hsp family was comprised of Hsp10, small hsp (sHsp), Hsp40, Hsp60, Hsp70/105, and Hsp90, with highest number of genes found within Hsp40 DnaJ homolog subfamily C members. Also, the differences in the orientation of the conserved motifs within Hsp family may have induced differences in transcriptional gene modulation in response to thermal stress in Brachionus koreanus. Overall, Hsp family-specific domains were highly conserved in all three Brachionus spp., relative to Homo sapiens and across other animal taxa and these findings will be helpful for future ecotoxicological studies focusing on Hsps.

2020-12-01T00:00:00Z Park, Yu Kyung Lee, Chang-Eun Lee, Hyoungseok Koh, Hye Yeon Kim, Sojin Lee, Sung Gu Kim, Jung Eun Yim, Joung Han Hong, Ju-Mi Kim, Ryeo-Ok Han, Se Jong Kim, Il-Chan https://repository.kopri.re.kr/handle/201206/11844 2022-03-24T07:14:40Z 2020-10-01T00:00:00Z

Title: The Effect of Translationally Controlled Tumor Protein (TCTP) of the Arctic Copepod Calanus glacialis on Protecting Escherichia coli Cells against Oxidative Stress Authors: Park, Yu Kyung; Lee, Chang-Eun; Lee, Hyoungseok; Koh, Hye Yeon; Kim, Sojin; Lee, Sung Gu; Kim, Jung Eun; Yim, Joung Han; Hong, Ju-Mi; Kim, Ryeo-Ok; Han, Se Jong; Kim, Il-Chan Abstract: Translationally controlled tumor protein (TCTP) is one of the most abundant proteins in various eukaryotic organisms. TCTPs play important roles in cell physiological processes, being implicated in cancer, cell proliferation, gene regulation, and heat shock response. TCTP is also considered an important factor in the resistance to oxidative stress induced by dithiothreitol or hydrogen peroxide (H2O2). The Arctic calanoid copepods have a variety of antioxidant defense systems to regulate the levels of potentially harmful reactive oxygen species generated by ultraviolet radiation in the Arctic marine ecosystem. However, information on the antioxidant activity of TCTP in the Arctic Calanus glacialis is still scarce. To understand the putative antioxidant function of the Arctic copepod C. glacialis TCTP (Cg-TCTP), its gene was cloned and sequenced. The Cg-TCTP comprised 522 bp and encoded a 174-amino acid putative protein with a calculated molecular weight of ~23 kDa. Recombinant Cg-TCTP (Cg-rTCTP) gene was overexpressed in Escherichia coli (BL21), and Cg-rTCTP-transformed cells were grown in the presence or absence of H2O2. Cg-rTCTP-transformed E. coli showed increased tolerance to high H2O2 concentrations. Therefore, TCTP may be an important antioxidant protein related to C. glacialis tolerance to oxidative stress in the harsh environment of the Arctic Ocean.

2020-10-01T00:00:00Z