https://repository.kopri.re.kr/handle/201206/11585 2026-04-26T02:01:46Z 2026-04-26T02:01:46Z Han, Tae Uk Kim, Jungwon Kim, Kitae https://repository.kopri.re.kr/handle/201206/13633 2022-07-08T00:54:33Z 2021-06-01T00:00:00Z

Title: Accelerated chromate reduction by tea waste: Comparison of chromate reduction properties between water and ice systems Authors: Han, Tae Uk; Kim, Jungwon; Kim, Kitae Abstract: The concentration of chromium (Cr) in natural water and soil environments has gradually increased in recent decades, owing to intensive use of Cr in industry and its subsequent disposal. In this study, we performed a comparison study on chromate (Cr6+) reduction by tea waste (green tea, black tea, red tea, and chamomile) in water (25 °C) and ice (-20 °C) to develop a new strategy for environmental-friendly stabilization of hazardous Cr6+ by freezing. This study shows that the freezing process can enhance the reduction of Cr6+ by tea waste. The residual Cr6+ concentration ratios (C/C0) by tea wastes in water were in the range of 0.71 (green tea) to 0.92 (chamomile); however, the ratios dramatically decreased under the freezing process (i.e., 0.06 by green tea, 0.13 by black tea, 0.18 by red tea, and 0.08 by green tea). According to the results obtained from the fluorescent, chromatographic, and spectroscopic analyses, under the freezing process, the enhanced reduction of Cr6+ could be explained by the freeze concentration of Cr6+, phenolic components in tea extracts, and protons in small liquid pockets in liquid-like layers (LLLs). In addition, the proposed system can efficiently purify the real Cr6+-containing wastewater (i.e., electroplating wastewater), indicating that the system will be economically feasible in cold regions (i.e., polar regions).

2021-06-01T00:00:00Z Ghimire, Nisha Han, So-Ra Kim, Byeollee Jung, Sang-Hee Park, Hyun Lee, Jun Hyuck Oh, Tae-Jin https://repository.kopri.re.kr/handle/201206/12969 2022-03-24T07:15:13Z 2021-05-01T00:00:00Z

Title: Complete genome sequencing and comparative CAZyme analysis of Rhodococcus sp. PAMC28705 and PAMC28707 provide insight into their biotechnological and phytopathogenic potential Authors: Ghimire, Nisha; Han, So-Ra; Kim, Byeollee; Jung, Sang-Hee; Park, Hyun; Lee, Jun Hyuck; Oh, Tae-Jin Abstract: Study of carbohydrate-active enzymes (CAZymes) can reveal information about the lifestyle and behavior of an organism. Rhodococcus species is well known for xenobiotic metabolism; however, their carbohydrate utilization ability has been less discussed till date. This study aimed to present the CAZyme analysis of two Rhodococcus strains, PAMC28705 and PAMC28707, isolated from lichens in Antarctica, and compare them with other Rhodococcus, Mycobacterium, and Corynebacterium strains. Genome-wide computational analysis was performed using various tools. Results showed similarities in CAZymes across all the studied genera. All three genera showed potential for significant polysaccharide utilization, including starch, cellulose, and pectin referring their biotechnological potential. Keeping in mind the pathogenic strains listed across all three genera, CAZymes associated to pathogenicity were analyzed too. Cutinase enzyme, which has been associated with phytopathogenicity, was abundant in all the studied organisms. CAZyme gene cluster of Rhodococcus sp. PAMC28705 and Rhodococcus sp. PAMC28707 showed the insertion of cutinase in the cluster, further supporting their possible phytopathogenic properties.

2021-05-01T00:00:00Z Shrestha, Prasansah Han, So-Ra Lee, Jun Hyuck Park, Hyun Oh, Tae-Jin https://repository.kopri.re.kr/handle/201206/13032 2022-03-24T07:14:49Z 2021-05-01T00:00:00Z

Title: A computational approach to identify CRISPR-Cas loci in the complete genomes of the lichen-associated Burkholderia sp. PAMC28687 and PAMC26561 Authors: Shrestha, Prasansah; Han, So-Ra; Lee, Jun Hyuck; Park, Hyun; Oh, Tae-Jin Abstract: The genus Burkholderia and its strains PAMC28687 and PAMC26561 are lichen-associated bacteria isolated from the Antarctic region. Our study is the first to provide the genome sequence of the Burkholderia sp. PAMC26561 strain. The genus Burkholderia includes bacteria that are pathogenic to plants, animals, and humans. Computational analysis of complete genomes of strains from the uncategorized Burkholderia group was performed using the NCBI databank and PATRIC (for genome sequence information) and CRISPRCasFinder (online and offline versions) software in order to predict the CRISPR loci and Cas genes. The RNAfold Webserver online software was used to predict RNA secondary structures. Our study showed that strain MSMB0852 (plasmid) possesses CRISPR-Cas system Class 2, and two lichen-associated strains, PAMC28687 (chromosome I) and PAMC26561 (chromosome I), possess CRISPR-Cas system Class 1. Additionally, only the two lichen-associated strains possess a variety of Cas genes.

2021-05-01T00:00:00Z Jo, Euna Lee, Seung Jae Choi, Eunkyung Kim, Jinmu Lee, Sung Gu Lee, Jun Hyuck Kim, Jeong-Hoon Park, Hyun https://repository.kopri.re.kr/handle/201206/12970 2022-03-24T07:15:29Z 2021-03-10T00:00:00Z

Title: Whole genome survey and microsatellite motif identification of Artemia franciscana Authors: Jo, Euna; Lee, Seung Jae; Choi, Eunkyung; Kim, Jinmu; Lee, Sung Gu; Lee, Jun Hyuck; Kim, Jeong-Hoon; Park, Hyun Abstract: The genus Artemia (Crustacea: Branchiopoda: Anostraca), known as brine shrimp, is an aquatic invertebrate living mainly in salt lakes. To date, seven species have been assigned to the genus Artemia excluding the parthenogenetic populations called Artemia parthenogenetic a [1]. Artemia species are important in aquaculture industry because their dormant cysts are easily hatched, and the nauplii can be used as a nutrient-rich food for fish [2,3]. In addition, they are also widely used as aquatic model organisms for ecotoxicity tests, along with Daphnia [4?6]. However, despite the significance of Artemia in aquacul ABSTRACT Artemia is an industrially important genus used in aquaculture as a nutritious diet for fish and as an aquatic model organism for toxicity tests. However, despite the significance of Artemia, genomic research remains incomplete and knowledge on its genomic characteristics is insufficient. In particular, Artemia franciscana of North America has been widely used in fisheries of other continents, resulting in invasion of native species. Therefore, studies on population genetics and molecular marker development as well as morphological analyses are required to investigate its population structure and to discriminate closely related species. Here, we used the Illumina Hi-Seq platform to estimate the genomic characteristics of A. franciscana through genome survey sequencing (GSS). Further, simple sequence repeat (SSR) loci were identified for microsatellite marker development. The predicted genome size was ?867 Mb using K-mer (a sequence of k characters in a string) analysis (K = 17), and heterozygosity and duplication rates were 0.655 and 0.809%, respectively. A total of 421467 SSRs were identified from the genome survey assembly, most of which were dinucleotide motifs with a frequency of 77.22%. The present study will be a useful basis in genomic and genetic research for A. franciscana.

2021-03-10T00:00:00Z