https://repository.kopri.re.kr/handle/201206/12731 2026-04-18T09:54:13Z 2026-04-18T09:54:13Z Moonsu Kim Huiji Kim Sun Young Moon Seong-Ah Shin Minji Kim Seyeon Choi Sugeun Gwon Lee, Jun Hyuck Hyun Ho Park Chang Sup Lee https://repository.kopri.re.kr/handle/201206/16442 2025-11-06T08:27:16Z 2025-01-01T00:00:00Z

Title: 3-bromo-4-(2-hydroxyethyl)-7-methoxy-2H-chromen-2-one inhibits allergic reactions in IgE-mediated RBL-2H3 cells and a passive cutaneous anaphylaxis mouse model Authors: Moonsu Kim; Huiji Kim; Sun Young Moon; Seong-Ah Shin; Minji Kim; Seyeon Choi; Sugeun Gwon; Lee, Jun Hyuck; Hyun Ho Park; Chang Sup Lee Abstract: Allergic reactions occur when the immune system overreacts to generally harmless substances, leading to both acute and chronic diseases, which can be fatal. Mast cells are critical mediators of allergic reactions as they bind allergens and trigger the release of inflammatory mediators. In this study, we investigated the anti-allergic effects of the coumarin derivative 3-bromo-4-(2-hydroxyethyl)-7-methoxy-2H-chromen-2-one in rat basophilic leukemia (RBL)-2H3 cells sensitized to dinitrophenyl (DNP)-immunoglobulin E (IgE) and human serum albumin (HSA). Our results demonstrated that 3-bromo-4-(2-hydroxyethyl)-7-methoxy-2H-chromen-2-one effectively reduces the release of β-hexosaminidase and histamine, inhibiting mast cell degranulation. Additionally, 3-bromo-4-(2-hydroxyethyl)-7-methoxy-2H-chromen-2-one suppressed the production of allergy-related pro-inflammatory cytokines (IL-4, IL-13, and TNF-α) and inhibited key signaling pathways, including MAPK, AKT, and NF-κB. Furthermore, in a passive cutaneous anaphylaxis (PCA) mouse model, 3-bromo-4-(2-hydroxyethyl)-7-methoxy-2H-chromen-2-one reduced ear edema and Evans blue infiltration, further confirming its anti-allergic effects. Collectively, these findings suggest that 3-bromo-4-(2-hydroxyethyl)-7-methoxy-2H-chromen-2-one is a promising candidate for the development of anti-allergic therapeutics.

2025-01-01T00:00:00Z Gwon Sugeun Shin Seong-Ah Kim Moonsu Moon Sun Young Choi Seyeon Kim Minji Lee, Jun Hyuck Park Hyun Ho Lee Chang Sup https://repository.kopri.re.kr/handle/201206/16441 2025-11-06T08:26:25Z 2025-01-01T00:00:00Z

Title: Anti-allergic effect of 5,7-dihydroxy-4-methylcoumarin in IgE-mediated RBL-2H3 cells and PCA murine model Authors: Gwon Sugeun; Shin Seong-Ah; Kim Moonsu; Moon Sun Young; Choi Seyeon; Kim Minji; Lee, Jun Hyuck; Park Hyun Ho; Lee Chang Sup Abstract: Allergy is an immune-mediated disorder characterized by an exaggerated response of the immune system to non-hazardous substances, resulting in allergic symptoms such as rash, itching, and runny nose. Current therapeutic interventions include antihistamines and steroids; however, they induce several side effects. Although 5,7-dihydroxy-4-methylcoumarin, a phytochemical derivative, has been demonstrated to exhibit antioxidant, anti-apoptotic, and anti-aggregatory effects, its anti-allergic properties and underlying molecular mechanisms remain elusive. Therefore, this study was conducted to investigate the anti-allergic effects of 5,7-dihydroxy-4-methylcoumarin in two experimental models: rat basophilic leukemia-2H3 cells sensitized using dinitrophenyl-specific immunoglobulin E (IgE)/human serum albumin and a passive cutaneous anaphylaxis (PCA) murine model. Our findings demonstrated that 5,7-dihydroxy-4-methylcoumarin reduced the release of histamine and beta-hexosaminidase and downregulated the mRNA expression of allergic-inflammatory cytokines, such as interleukin (IL)-4, IL-13, and tumor necrosis factor-alpha, as well as the inflammatory enzyme cyclooxygenase-2. Furthermore, 5,7-dihydroxy-4-methylcoumarin reduced the phosphorylation of mitogen-activated protein kinases such as extracellular signal-regulated kinase and p38, as well as protein kinase B. In vivo, 5,7-dihydroxy-4-methylcoumarin reduced PCA reaction, as evidenced by reduced Evans blue dye extravasation in IgE-mediated local allergic responses. Collectively, these results suggest that 5,7-dihydroxy-4-methylcoumarin holds promise as a novel candidate for the development of anti-allergic drugs.

2025-01-01T00:00:00Z Han, Duk Ki https://repository.kopri.re.kr/handle/201206/14581 2023-08-17T07:01:09Z 2023-07-26T00:00:00Z

Title: Microbial Paleogenomics using marine sediment in the Antarctic Ocean Authors: Han, Duk Ki

2023-07-26T00:00:00Z Jung, Yoon Taek park, sangeun Kim, Hyun-cheol https://repository.kopri.re.kr/handle/201206/14584 2023-08-16T00:31:11Z 2023-01-01T00:00:00Z

Title: Observation of spatial and temporal patterns of seasonal ground deformation in central Yakutia using time series InSAR data in the freezing season Authors: Jung, Yoon Taek; park, sangeun; Kim, Hyun-cheol Abstract: The seasonal freeze/thaw variations of the active layer cause seasonal ground deformation, such as thaw subsidence in summer and frost heave in winter due to the cyclic phase changes of soil water. The Synthetic Aperture Radar (SAR) remote sensing, especially interferometric SAR (InSAR) techniques, has been proven to be a very useful tool for observing surface displacements of permafrost environments. Most previous studies on seasonal InSAR displacement have been concentrated in several study sites in the Arctic tundra regions. The objective of this study is to extend the InSAR analysis of seasonal ground deformations to boreal forest regions, where monitoring ground deformation is particularly important in terms of better understanding active layer dynamics under various ecological and geological conditions. In this study, we presented a strategy to analyze the time series SAR data in the freezing season to estimate the differential phase for seasonal ground deformation while overcoming the problem of coherence losses due to the rapid growth of vegetation during the short growing season. We applied the SBAS-based InSAR processing method to Sentinel-1 SAR in the central Yakutian lowlands, where boreal forests and thermokarst landforms are widely distributed, and retrieved frost-related displacement signals successfully. In addition to the magnitude of ground deformation in the freezing season, we further examined the temporal deformation pattern related to the progression of the freezing front in the soil during active freezing. Based on the Stefan solution, we proposed a piecewise linear model for the time series deformation. The proposed model divides the time series displacement according to the progression of freezing into three linear segments, and the slope parameter of each linear model can be interpreted as indicating the hydrological characteristics of the upper, middle, and lower parts of the soil. The results for the Yakutian lowlands illustrated the potential of SAR remote sensing to observe and quantify spatial details of the thermal and hydrological properties inside the active layer soil even in highly vegetated subarctic boreal forest areas.

2023-01-01T00:00:00Z