https://repository.kopri.re.kr/handle/201206/5241 2026-04-21T10:19:46Z 2026-04-21T10:19:46Z Kim, Ji Hee https://repository.kopri.re.kr/handle/201206/9060 2022-03-24T07:14:45Z 2015-09-15T00:00:00Z

Title: The 2nd Korean Antarctic Research Station Construction Authors: Kim, Ji Hee

2015-09-15T00:00:00Z Baek, Kiwoon Lee, Hong Kum Kim, Ji Hee Hong, Soon Gyu Kim, Mi-Kyeong Jung, You-Jung Park, Hyun Hwang, Chung Yeon Lee, Yung Mi https://repository.kopri.re.kr/handle/201206/6709 2022-03-24T07:11:37Z 2015-01-01T00:00:00Z

Title: Psychroserpens jangbogonensis sp. nov., apsychrophilic bacterium isolated from Antarcticmarine sediment Authors: Baek, Kiwoon; Lee, Hong Kum; Kim, Ji Hee; Hong, Soon Gyu; Kim, Mi-Kyeong; Jung, You-Jung; Park, Hyun; Hwang, Chung Yeon; Lee, Yung Mi Abstract: A Gram-staining-negative, yellow-pigmented, aerobic, rod-shaped and non-motile bacterium, PAMC 27130T, was isolated from the marine sediment of the Ross Sea, Antarctica. The temperature, pH and NaCl tolerance ranges for growth were 4？20 6C, pH 6.0？9.0 and 0.5？ 5.0% (w/v) NaCl, respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PAMC 27130T belonged to the genus Psychroserpens and was closely related to Psychroserpens mesophilus, Psychroserpens damuponensis and Psychroserpens burtonensis with 97.2, 94.7 and 94.2% sequence similarities, respectively. Genomic relatedness analyses based on average nucleotide identity and genome-to-genome distance showed that strain PAMC 27130T could be clearly distinguished from other species of the genus Psychroserpens . The genomic DNA G+C content was 32.7 mol%. The major fatty acids (.10 %) were C20 : 4v6c (13.2 %), iso-C15 : 0 (12.3 %), iso-C15 : 1 G (11.7 %) and iso-C15 : 0 3-OH (10.0 %). The major respiratory isoprenoid quinone was menaquinone-6 (MK-6) and the polar lipids were phosphatidylethanolamine, two unidentified aminolipids, an unidentified phospholipid, an unidentified aminophospholipid and three unidentified lipids. On the basis of genotypic and phenotypic data collected in this study, it is proposed that strain PAMC 27130T represents a novelspecies of the genus Psychroserpens, for which the name Psychroserpens jangbogonensis sp. nov. is proposed. The type strain is PAMC 27130T (5KCTC 42128T5JCM 30228T).

2015-01-01T00:00:00Z Kim, Mincheol Kim, Ok-Sun Ahn, Tae Seok Choi, Taejin Lee, Joohan Kim, Ji Hee Hong, Soon Gyu Lim, Hyoun Soo Cho, Ahnna https://repository.kopri.re.kr/handle/201206/7303 2022-03-24T07:12:51Z 2015-01-01T00:00:00Z

Title: Highly heterogeneous soil bacterial communities around Terra Nova Bay of Northern Victoria Land, Antarctica Authors: Kim, Mincheol; Kim, Ok-Sun; Ahn, Tae Seok; Choi, Taejin; Lee, Joohan; Kim, Ji Hee; Hong, Soon Gyu; Lim, Hyoun Soo; Cho, Ahnna Abstract: Given the diminished role of biotic interactions in soils of the continental Antarctica, abiotic factors have been believed to be the most prevailing parameter in structuring microbial communities. Many ice-free regions still remain unexplored and little is understood about which environmental gradients dominate the variation of bacterial communities. In this study, we investigated the soil bacterial community around Terra Nova Bay of Victoria Land using pyrosequencing and determined which environmental variables govern the bacterial community structure at the local scale. Six bacterial phyla such as Actinobacteria, Proteobacteria, Acidobacteria, Chloroflexi, Cyanobacteria, and Bacteroidetes were dominant but their relative abundance greatly varied across locations. Bacterial community structures were little affected by spatial distance but structured more strongly by site difference which parallels to the variation of soil physicochemical compositions. At both phylum- and species levels, bacterial community structure was explained most by pH and water content and to a lesser extent certain earth elements and trace metals also had important roles in shaping community variation. The higher level of heterogeneity of bacterial community structure found in this local site indicates how soil bacterial communities have been distinctively adapted to different compositions of edaphic variables under extreme environmental conditions. Taken together, these findings will greatly advance our understanding about how soil bacterial populations have been adapted to this harsh environment and how they will respond to changing climates in the future.

2015-01-01T00:00:00Z Lee, Inae Chun, Jongsik Lee, Hong Kum Kim, Ji Hee Hong, Soon Gyu Baek, Kiwoon Cho, Yirang Jung, You-Jung Hwang, Chung Yeon Lee, Yung Mi https://repository.kopri.re.kr/handle/201206/6705 2022-03-24T07:10:49Z 2014-01-01T00:00:00Z

Title: Lacinutrix jangbogonensis sp. nov., a psychrophilic bacterium isolated from Antarctic marine sediment and emended description of the genus Lacinutrix Authors: Lee, Inae; Chun, Jongsik; Lee, Hong Kum; Kim, Ji Hee; Hong, Soon Gyu; Baek, Kiwoon; Cho, Yirang; Jung, You-Jung; Hwang, Chung Yeon; Lee, Yung Mi Abstract: A Gram-negative, strictly aerobic, non-motile, rod-shaped and psychrophilic bacterial strain, PAMC 27137T, was isolated from the marine sediment of the Ross Sea, Antarctica. Strain PAMC 27137T was observed to grow at 4-10？C, at pH 6.5-7.5 and in the presence of 2.5-4.0% (w/v) sea salts. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain PAMC 27137T belongs to the genus Lacinutrix showing the high similarities with Lacinutrix mariniflava JCM 13824T (97.6%) and Lacinutrix algicola JCM 13825T (97.1%). Genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strain PAMC 27137T is clearly distinguished from the most closely related Lacinutrix species. The major fatty acids (>5%) were identified as iso-C15:1 G (19.9%), iso-C15:0 (19.3%), iso-C17:0 3-OH (11.3%), summed feature 9 (C16:0 10-methyl and/or iso-C17:1 ω9c as defined by MIDI, 9.1%), iso-C15:0 3-OH (7.5%), and anteiso-C15:1 A (5.8%). The polar lipids were found to consist of phosphatidylethanolamine, an unidentified aminolipid, an unidentified aminophospholipid, and five unidentified phospholipids. The major respiratory quinone was identified as MK-6. The genomic DNA G+C content was determined to be 33.5 mol%. Based on the data from this polyphasic taxonomic study, strain PAMC 27137T is considered to represent a novel species of the genus Lacinutrix, for which the name Lacinutrix jangbogonensis sp. nov. is proposed. The type strain is PAMC 27137T (= KCTC 32573T = JCM 19883T).

2014-01-01T00:00:00Z