https://repository.kopri.re.kr/handle/201206/5279 2026-04-16T00:07:07Z 2026-04-16T00:07:07Z Lee, Hong Kum Kim, Sung Jin Yim, Joung Han https://repository.kopri.re.kr/handle/201206/5896 2022-03-24T07:11:49Z 2008-01-01T00:00:00Z

Title: Statistical Optimization of Medium Components for the Production of Prodigiosin by Hahella chejuensis KCTC 2396 Authors: Lee, Hong Kum; Kim, Sung Jin; Yim, Joung Han Abstract: Prodigiosin is a natural red pigment with algicidal activity against Cochlodinium polykrikoides, a major harmful redtide microalga. To increase the yield of prodigiosin production by Hahella chejuensis KCTC 2396, significant medium components were determined using a two-level Plackett- Burman statistical design technique. Among 12 components included in basal medium, NaHCO3, Na2SiO3, NH4NO3, Na2SO4, and CaCl2 were determined to be important for prodigiosin production. The medium formulation was finally optimized using a Box-Behnken design as follows: 1% sucrose;0.4% peptone;0.1% yeast extract;and (g/l): NaCl, 20.0;Na2SO4, 9.0;CaCl2, 1.71;KCl, 0.4;and (mg/l): H3BO3, 10.0;KBr, 50.0;NaF, 2.0;NaHCO3, 45.0;Na2SiO3, 4.5;NH4NO3, 4.5. The predicted maximum yield of prodigiosin in the optimized medium was 1.198 g/l by the Box-Behnken design, whereas the practical production was 1.495 g/l, which was three times higher than the basal medium (0.492 g/l).

2008-01-01T00:00:00Z Yim, Joung Han Lee, Hong Kum https://repository.kopri.re.kr/handle/201206/5938 2022-03-24T07:12:15Z 2004-01-01T00:00:00Z

Title: Axenic culture of Gyrodinium impudicum strain KG03, a marine red-tide microalga that produces exopolysaccharide Authors: Yim, Joung Han; Lee, Hong Kum Abstract: An exopolysaccharide-producing microalgal dinoflagellate was isolated from a red-tide bloom and designated strain KG03. A bacteria-free culture of strain KG03 was achieved using a modified wash with phototaxis and antibiotic treatment. Combined treatment with neomycin and cephalosporin was the most effective for eliminating the bacteria associated with the microalgae. Strain KG03 was identified as Gyrodinium impudicum by analyzing the ITS regions of the 5.8S rDNA, 18S rDNA, morphological phenotype and fatty acid composition. The exopolysaccharide production and cell growth in a 300-ml photobioreactor were increased 2.7- and 2.4-fold, respectively, compared with that in a flask culture at the first isolation step.

2004-01-01T00:00:00Z