https://repository.kopri.re.kr/handle/201206/5419 2026-04-05T00:48:10Z 2026-04-05T00:48:10Z Bang, Hyun Woo Kang, Sung-Ho Lee, Wonchoel https://repository.kopri.re.kr/handle/201206/5977 2022-03-24T07:12:02Z 2005-01-01T00:00:00Z

Title: Study on the community structure of meiofauna in Marian Cove, King George Island, Antarctica Authors: Bang, Hyun Woo; Kang, Sung-Ho; Lee, Wonchoel Abstract: The community structure, vertical distribution and harparcticoids composition of the meiofauna community were observed from five stations in Marian Cove, King George Island and one station on the northeaster side of Nelson Island.

2005-01-01T00:00:00Z Sung, Ki Cheol Yim, Joung Han Cho, Kyeung Hee Lee, Yoo Kyung Lee, Hong Kum Cho, Hyun Hee https://repository.kopri.re.kr/handle/201206/7565 2022-03-24T07:13:07Z 2005-01-01T00:00:00Z

Title: Diversity of Arctic & Antarctic Marine Bacteria Authors: Sung, Ki Cheol; Yim, Joung Han; Cho, Kyeung Hee; Lee, Yoo Kyung; Lee, Hong Kum; Cho, Hyun Hee Abstract: The Arctic & Antarctic are representative cold habitats that offer good sources of useful enzymes with activity at low temperature. Various marine bacterial strains were isolated from marine environment around the Korean Arctic Research Station Dasan located at Ny-Alesund, Svalbard, Norway (79°N, 12°E), and the King Sejong Station in Antarctica (62°S, 58°W). The collected samples were diluted in distilled seawater, and spread on marine agar plates. They cultured at 10℃, and bacterial isolates were preserved in glycerol media (15%, v/v) at – 80℃. Phylogenetic analysis of 16S rDNA sequences indicated that the marine bacteria belonged to alpha-, beta-, and gamma-Proteobacteria, the CFB group, and High GC Gram-positive bacteria. They were Aequorivita, Ahrensia, Algoriphagus, Carnobacterium, Cellulophaga, Cobetia, Colwellia, Exiguobacterium, Flavobacterium, Glaciecola, Hydrogenophaga, Hyphomicrobium, Leifsonia, Loktanella, Marinobacter, Marinomonas, Paracoccus, Pibocella, Plantibacter, Planococcus, Polaribacter, Pseudoaltermonas, Pseudomonas, Psychrobacter, Psychroserpens, Roseobacter, Shewanella, Sulfitobacter, Vibrio and Zobellia species. Among the bacteria, we found several candidates for new species or genus. We also screened chitinase-, lipase-, or protease-producing bacteria. Most of them belonged to genus Pseudoalteromonas. We expect that these Arctic & Antarctic bacteria can be used to develop new industrial enzymes.

2005-01-01T00:00:00Z 이재형 Lee, Yoo Kyung Lee, Hong Kum https://repository.kopri.re.kr/handle/201206/7566 2022-03-24T07:13:44Z 2005-01-01T00:00:00Z

Title: Molecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027 Authors: 이재형; Lee, Yoo Kyung; Lee, Hong Kum Abstract: The chiK gene encoding chitinase ChiK was isolated from a marine bacterium, Vibrio sp. 98CJ11027. We amplified a partial sequence of the chiK using chitinase-specific primer sets. Using inverse PCR cloning method with two specific sets of PCR primers rather than single set, we identified the 4 kb DNA fragment containing chiK gene. From the fragment, we amplified the full chiK gene and cloned into pUC18 vector designated as pChiK. The recombinant E. coli clones harboring pChiK produced a clear zone around the colony in the colloidal chitin plates. Open reading frame of the chiK is 2553 bp long encoding a ChiK precursor protein of 851 amino acids, which consist of N-terminal signal peptide of 21 amino acids and a mature ChiK protein with 830 amino acids. The deduced amino acid sequences of chiK shows 89% identity with that of Vibrio carchariae chitinase. The conserved module of Vibrio sp. 98CJ11027 ChiK indicates that the ChiK belongs to the family 18 of glycoside hydrolases.

2005-01-01T00:00:00Z 홍성수 Kang, Sung-Ho Kang, Jae-Shin https://repository.kopri.re.kr/handle/201206/7567 2022-03-24T07:13:50Z 2005-01-01T00:00:00Z

Title: Optical culture conditions of Antarctic diatom Chaetoceros neogracile Authors: 홍성수; Kang, Sung-Ho; Kang, Jae-Shin Abstract: 남극산 해양 규조류의 최적 성장요인 실험. 규조 성장에 영향을 미치는 영양염 농도 및 온도 구배에 따른 성장조건 조사.

2005-01-01T00:00:00Z