DSpace Collection:https://repository.kopri.re.kr/handle/201206/98412026-04-27T01:33:13Z2026-04-27T01:33:13ZChromosomallevel assembly of Takifugu obscurus (Abe, 1949) genome using thirdgeneration DNA sequencing and HiC analysisKang, SeunghyunKim, Jin-HyoungJo, EunaLee, Seung JaeJeong, JihyeKim, Bo-MiLee, Jun HyuckOh, Tae-JinYum, SeungshicRhee, Jae-SungPark, Hyunhttps://repository.kopri.re.kr/handle/201206/117682022-03-24T07:14:50Z2020-03-01T00:00:00ZTitle: Chromosomallevel assembly of Takifugu obscurus (Abe, 1949) genome using thirdgeneration DNA sequencing and HiC analysis
Authors: Kang, Seunghyun; Kim, Jin-Hyoung; Jo, Euna; Lee, Seung Jae; Jeong, Jihye; Kim, Bo-Mi; Lee, Jun Hyuck; Oh, Tae-Jin; Yum, Seungshic; Rhee, Jae-Sung; Park, Hyun
Abstract: The Tetraodontidae family are known to have relatively small and compact genomes compared to
other vertebrates. The obscure puffer fish Takifugu obscurus is an anadromous species that migrates
to freshwater from the sea for spawning. Thus the euryhaline characteristics of T. obscurus have been
investigated to gain understanding of their survival ability, osmoregulation, and other homeostatic
mechanisms in both freshwater and seawater. In this study, a high-quality chromosome-level
reference genome for T. obscurus was constructed using long-read Pacific Biosciences (PacBio)
Sequel sequencing and a HiCbased chromatin contact map platform. The final genome assembly of
T. obscurus is 381 Mb, with a contig N50 length of 3,296 kb and longest length of 10.7 Mb, from a
total of 62 Gb of raw reads generated using singlemolecule realtime sequencing technology from a
PacBio Sequel platform. The PacBio data were further clustered into chromosome-scale scaffolds
using a HiC approach, resulting in a 373 Mb genome assembly with a contig N50 length of 15.2 Mb
and and longest length of 28 Mb. When we directly compared the 22 longest scaffolds of T. obscurus
to the 22 chromosomes of the tiger puffer Takifugu rubripes, a clear one-to-one orthologous
relationship was observed between the two species, supporting the chromosome-level assembly of T.
obscurus. This genome assembly can serve as a valuable genetic resource for exploring fugu-specific
compact genome characteristics, and will provide essential genomic information for understanding
molecular adaptations to salinity fluctuations and the evolution of osmoregulatory mechanisms2020-03-01T00:00:00Z