https://repository.kopri.re.kr/handle/201206/12121 Acamedy-Research-Industry Joint Programs for Polar Research Promotion 2026-04-22T22:31:57Z https://repository.kopri.re.kr/handle/201206/16035 Title: Longitudinal gut microbiota dynamics in Antarctic research mission crews Authors: Lee, Min-Jung; Lee, Seung-Hwan; Min, Huitae; Nam, Tae-Wook; Hong, Soon Gyu; Oh, Bumjo; Kim, Joo Hyeong; Kim, Yeon-Ran; Kim, Bong-Soo; Seok, Yeong-Jae Abstract: Introduction A prolonged stay at Antarctic research stations poses unique challenges due to extreme environmental conditions, restricted diets, and cold temperatures, all of which can influence the gut microbiota, an important factor in host health. However, our understanding of how the Antarctic environment affects the gut microbiota remains limited due to small cohort sizes and short study durations.Methods We analyzed 467 fecal samples collected longitudinally from 48 participants who stayed at Antarctic stations for up to 16 months.Results Before departing to the Antarctic bases, male participants exhibited three distinct types of gut microbiota, which were differentially altered during and after the stay, depending on the pre-existing microbiota type. Prevotella-dominant microbiota was more susceptible to environmental changes, including the diet, compared with Bacteroides-dominant microbiota. Although the dominant genera in the gut microbiota were stable across all microbiota types, minor genera with high variability could mediate changes in the microbiota. Sharing diets and having frequent contact resulted in cohabitation effects among genetically unrelated participants in the extremely isolated Antarctic environment. Although taxonomic composition shifted in response to the Antarctic environment, predicted functions of the gut microbiota remained relatively stable.Discussion This study reveals that long-term residence in Antarctic research stations alters the gut microbiota in ways that depends on the intrinsic microbiota prior to the mission. These findings enhance our understanding of human gut microbiota adaptation under extreme and isolated environmental conditions. 2025-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/16442 Title: 3-bromo-4-(2-hydroxyethyl)-7-methoxy-2H-chromen-2-one inhibits allergic reactions in IgE-mediated RBL-2H3 cells and a passive cutaneous anaphylaxis mouse model Authors: Moonsu Kim; Huiji Kim; Sun Young Moon; Seong-Ah Shin; Minji Kim; Seyeon Choi; Sugeun Gwon; Lee, Jun Hyuck; Hyun Ho Park; Chang Sup Lee Abstract: Allergic reactions occur when the immune system overreacts to generally harmless substances, leading to both acute and chronic diseases, which can be fatal. Mast cells are critical mediators of allergic reactions as they bind allergens and trigger the release of inflammatory mediators. In this study, we investigated the anti-allergic effects of the coumarin derivative 3-bromo-4-(2-hydroxyethyl)-7-methoxy-2H-chromen-2-one in rat basophilic leukemia (RBL)-2H3 cells sensitized to dinitrophenyl (DNP)-immunoglobulin E (IgE) and human serum albumin (HSA). Our results demonstrated that 3-bromo-4-(2-hydroxyethyl)-7-methoxy-2H-chromen-2-one effectively reduces the release of β-hexosaminidase and histamine, inhibiting mast cell degranulation. Additionally, 3-bromo-4-(2-hydroxyethyl)-7-methoxy-2H-chromen-2-one suppressed the production of allergy-related pro-inflammatory cytokines (IL-4, IL-13, and TNF-α) and inhibited key signaling pathways, including MAPK, AKT, and NF-κB. Furthermore, in a passive cutaneous anaphylaxis (PCA) mouse model, 3-bromo-4-(2-hydroxyethyl)-7-methoxy-2H-chromen-2-one reduced ear edema and Evans blue infiltration, further confirming its anti-allergic effects. Collectively, these findings suggest that 3-bromo-4-(2-hydroxyethyl)-7-methoxy-2H-chromen-2-one is a promising candidate for the development of anti-allergic therapeutics. 2025-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/16441 Title: Anti-allergic effect of 5,7-dihydroxy-4-methylcoumarin in IgE-mediated RBL-2H3 cells and PCA murine model Authors: Gwon Sugeun; Shin Seong-Ah; Kim Moonsu; Moon Sun Young; Choi Seyeon; Kim Minji; Lee, Jun Hyuck; Park Hyun Ho; Lee Chang Sup Abstract: Allergy is an immune-mediated disorder characterized by an exaggerated response of the immune system to non-hazardous substances, resulting in allergic symptoms such as rash, itching, and runny nose. Current therapeutic interventions include antihistamines and steroids; however, they induce several side effects. Although 5,7-dihydroxy-4-methylcoumarin, a phytochemical derivative, has been demonstrated to exhibit antioxidant, anti-apoptotic, and anti-aggregatory effects, its anti-allergic properties and underlying molecular mechanisms remain elusive. Therefore, this study was conducted to investigate the anti-allergic effects of 5,7-dihydroxy-4-methylcoumarin in two experimental models: rat basophilic leukemia-2H3 cells sensitized using dinitrophenyl-specific immunoglobulin E (IgE)/human serum albumin and a passive cutaneous anaphylaxis (PCA) murine model. Our findings demonstrated that 5,7-dihydroxy-4-methylcoumarin reduced the release of histamine and beta-hexosaminidase and downregulated the mRNA expression of allergic-inflammatory cytokines, such as interleukin (IL)-4, IL-13, and tumor necrosis factor-alpha, as well as the inflammatory enzyme cyclooxygenase-2. Furthermore, 5,7-dihydroxy-4-methylcoumarin reduced the phosphorylation of mitogen-activated protein kinases such as extracellular signal-regulated kinase and p38, as well as protein kinase B. In vivo, 5,7-dihydroxy-4-methylcoumarin reduced PCA reaction, as evidenced by reduced Evans blue dye extravasation in IgE-mediated local allergic responses. Collectively, these results suggest that 5,7-dihydroxy-4-methylcoumarin holds promise as a novel candidate for the development of anti-allergic drugs. 2025-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/15441 Title: Compensatory mechanisms of Antarctic marine benthic animals in response to climate change Authors: Kim, Taewon 2024-05-08T00:00:00Z