https://repository.kopri.re.kr/handle/201206/15770 2026-04-16T22:19:17Z https://repository.kopri.re.kr/handle/201206/16501 Title: Draft genome and de novo transcriptome assembly of the Antarctic marine flatworm Obrimoposthia wandeli Authors: Shin, Seung Chul; Kim, Sanghee Abstract: Objectives Obrimoposthia wandeli is the most abundant marine planarian in the intertidal zone of the maritime Antarctic region and is presumed to have adapted to the extreme conditions of the Southern Ocean. However, genomic studies on marine flatworms remain extremely limited, with only one marine planarian genome currently available in public databases. We present the first draft genome and de novo transcriptome assembly of O. wandeli from Antarctica, providing a valuable genomic resource for the study of flatworm biology and environmental adaptation under harsh environments. Data description We sequenced the genome and transcriptome of O. wandeli collected near King Sejong Station using Oxford Nanopore long-read, Illumina paired-end, and RNA sequencing. The draft genome assembly spans 1.35 Gb across 6,912 contigs, with an N50 of 343,088 bp and an estimated 48,310 predicted genes. The assembly showed Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness scores of 80.2% (genome) and 80.8% (proteins). The de novo transcriptome assembly identified 26,169 non-redundant transcripts, with a BUSCO completeness of 93.1%. These genomic and transcriptomic datasets represent the first Antarctic marine planarian reference and provide a valuable resource for studying marine flatworm genomics. 2025-12-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/16604 Title: Rhodococcus aromaticivorans sp. nov., an o-xylene degrading bacterium, and evidence supporting reclassification of Rhodococcus jostii RHA1 Authors: Neak Muhammad; Jeon, Jehyun; Kim Eungbin; Kim, Dockyu; Lee, Yung Mi Abstract: A Gram-positive, aerobic, non-motile bacterium with a rod-coccus shape, designated DK17(T), was isolated from a crude oil-contaminated soil and identified as a member of the genus Rhodococcus based on 16S rRNA gene analysis, showing highest similarity (99.93%) to Rhodococcus jostii DSM 44719(T). However, average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain DK17(T) and type strains within the genus Rhodococcus were below the species delineation thresholds of 95% and 70%, respectively. In contrast, DK17(T) exhibited ANI and dDDH values over 99% and 92%, respectively, with R. jostii RHA1. Comparative genomic analysis revealed that DK17(T) and RHA1 shared 93.5% of genes, while RHA1 and R. jostii NBRC 16295T shared only 78.6%, indicating a closer relationship between DK17(T) and RHA1. Both strains possess large genomes (similar to 9.5-9.7 Mb) comprising a linear chromosome and multiple plasmids, and encode multiple dioxygenases and secondary metabolite biosynthetic gene clusters. In vitro assays confirmed o-xylene degradation by both DK17(T) and R. jostii RHA1, consistent with the presence of the akb gene cluster. Both strains shared C16:0 as a major fatty acid and menaquinone-8 (H2) as the dominant quinone. Based on genomic, phenotypic, and chemotaxonomic data, DK17(T) (=KCCM 90599(T) = InaCC B1662(T)) is proposed as a novel species, Rhodococcus aromaticivorans sp. nov., and R. jostii RHA1 is reclassified as a member of the same species. 2025-12-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/16602 Title: Historical Review & Implications of Korea’s Arctic Sea Route Policy Authors: Seo, Hyun Kyo; 박종관 Abstract: 우리나라는 2013년 북극이사회 옵서버 지위에 오른 후 같은 해 12월 한국 최초의 북극정책인 ‘북극정책기본계획’을 수립하였고, 여기에 북극항로 세부과제가 포함되면서 북극항로 정책의 첫발을 내디뎠다. 이어, 2018년 ‘북극활동진흥기본계획’(’18-’22)에서도 북극항로 관련 과제는 2017년 문재인 정부의 ‘나인브릿지(9-Bridge) 전략’을 이행할 실행과제로서 그 의미를 더하였다. 이어 2021년 정부는 극지활동진흥법 제정 후, 동 법 6조에 근거하여 우리나라 최초의 북극 관련 법정 기본계획인 제1차 극지활동진흥기본계획(’23-’27)을 수립·발표했다. 이 기본계획부터는 해운·물류루트로서의 북극항로부터 조선, 항만, 기술개발 등 인프라 구축까지 모두 북극항로 아젠다에 포괄적으로 포함됐고, 현재 이재명 정부에서는 ‘북극항로’ 아젠다를 123대 국정과제 최우선 과제로 채택했다. 본 연구는 북극항로 관련 국내·외 선행연구를 분석하고, 북극정책기본계획부터 이재명 정부 시기까지 시대별로 북극항로 정책을 분석하였다. 그리고, 북극정책 모형(2019)을 기반으로 이재명 정부의 북극항로 국정과제 내 세부과제들을 재분류하여 국제협력, 기반구축, 과학연구 등의 부문에서 추가 세부과제들을 제안하였다. 그래서 향후 북극항로 기본계획 수립 시 세부과제 개발 방안 및 바람직한 북극항로 정책 방향을 제안하였다. 2025-12-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/16610 Title: Genetic Evidence of Active Circulation and Evolution of Diverse Penguin Siadenoviruses in Antarctica Based on Partial DNA Sequences Authors: Lee, Sook-Young; Kim, Sanghee; Kim, Ji Hee; Kim, Jong-U; Kim, Jeong-Hoon; Kim, Jihee; Oh, Younggeun; Song, Jin-Won Abstract: Antarctica, one of the most isolated and extreme regions on Earth, hosts diverse bird species that share breeding and feeding habitats, facilitating interspecies transmission of pathogens. In this study, we investigated penguin siadenoviruses using cloacal swab samples collected from Antarctic penguins between 2017 and 2023 to explore their genetic diversity and evolutionary relationships. The complete hexon gene was obtained from Adelie penguins, while partial hexon and DNA polymerase sequences were detected in Adelie, Chinstrap, and Gentoo penguins. Phylogenetic and molecular analysis identified multiple siadenoviruses classified into two distinct lineages, indicating ongoing viral evolution in this region. The hexon genes exhibited considerable genetic diversity caused by recombination and mutation, and predicted hypervariable regions―targets of neutralizing antibodies―showed significant structural differences among penguin siadenoviruses. These finding suggests that penguin siadenoviruses are not restricted to a single host species but may circulate among various penguin populations across the Antarctic region. This implies potential cross-infection between local and surrounding penguin populations. Although the predicted structural models showed limited accuracy due to the use of distant templates, the genetic and structural differences observed provide valuable insights into the adaptive evolution of these viruses. Our findings provide an important foundation for understanding viral transmission and evolution in Antarctic avifauna. Furthermore, findings from this study may guide early detection and risk assessment of emerging viral threats in Antarctica. 2025-11-01T00:00:00Z