https://repository.kopri.re.kr/handle/201206/5082 2026-04-16T00:08:58Z https://repository.kopri.re.kr/handle/201206/6179 Title: Selection of Extraction Solvent and Temperature Effect on Stability of Algicidal Agent Prodigiosin Authors: Lee, Sung Gu; Park, Heeyong; Han, Se Jong; Yim, Joung Han; Kim, Tai Kyoung Abstract: To develop prodigiosin as a biological control agent against Chattonella antiqua, a harmful alga that can cause red tides, selection of an organic solvent for prodigiosin extraction from culture broth and a test to determine the long-term stability of prodigiosin were performed. Prodigiosin was extracted using 9 solvents, and the extracts were analyzed by LC-MS. Acetone was selected as the best organic solvent because of its high extraction efficiency and less process time. Stability tests for prodigiosin were performed at various temperatures, and algicidal activity against C. antiqua was also tested. Ultimately, more than 98% stability was sustained after 30 days at 4℃, and less than 30% stability was maintained after 30 days at 37℃. Although prodigiosin was kept for 30 days in an optimum organic solvent, the stability of prodigiosin was safely maintained and algicidal activity was sustained at low temperatures such as 4℃. Considering these results, we know that acetone was a very useful extraction and storage solvent for the biological control agent, prodigiosin.rm stability of prodigiosin were performed. Prodigiosin was extracted using 9 solvents, and the extracts were analyzed by LC-MS. Acetone was selected as the best organic solvent because of its high extraction efficiency and less process time. Stability tests for prodigiosin were performed at various temperatures, and algicidal activity against C. antiqua was also tested. Ultimately, more than 98% stability was sustained after 30 days at 4℃, and less than 30% stability was maintained after 30 days at 37℃. Although prodigiosin was kept for 30 days in an optimum organic solvent, the stability of prodigiosin was safely maintained and algicidal activity was sustained at low temperatures such as 4℃. Considering these results, we know that acetone was a very useful extraction and storage solvent for the biological control agent, prodigiosin. 2012-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/8439 Title: Effects of Temperature and Solvents on the Stability of Algicidal Agent Prodigiosin Authors: Park, Heeyong; Yim, Joung Han; Han, Se Jong; Kim, Tai Kyoung; Lee, Sung Gu Abstract: To develop prodigiosin as biological control agent against Chattonella antiqua, a harmful alga that can cause red tides, selection of an organic solvent for prodigiosin extraction from culture broth and a test to determine the stability of prodigiosin were performed. Prodigiosin was extracted using nine solvents, and extracts were analyzed by LC-MS. Acetone was selected as the best organic solvent because of its high extraction efficiency and less process time. Stability tests for progidiosin were performed at various temperatures, and algicidal activity against C. antiqua was also tested. Ultimately, more than 98% stability was sustained after 30 days at 4℃, and less than 30% stability was maintained after 30 days at 37℃. More than 5~14% of the algicidal activity of prodigiosin extracted with acetone was sustained at each temperature, when compared with the prodigiosin extracted with ethanol. Although prodigiosin was kept for 30 days in an optimum organic solvent, the stability of prodigiosin was safely maintained and algicidal activity was sustained at low temperatures such as 4℃. Considering these results, we know that acetone was a very useful extraction agent for the extraction of prodigiosin as a biological control agent. [This work was supported by grants from the Korea Polar Research Institute (PE11060 and PN07020).] 2012-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/8011 Title: Selection of an optimal organic solvent for the extraction of prodigiosin Authors: Park, Heeyong; Han, Se Jong; Yim, Joung Han; Lee, Sung Gu Abstract: To develop prodigiosin as biological control agent against Chattonella antiqua, a harmful alga that can cause red tides, selection of an organic solvent for prodigiosin extraction from culture broth was performed. Prodigiosin was extracted using nine solvents (hexane, toluene, chloroform, ethyl acetate, ethanol, methanol, acetone, acetonitrile, and seawater), and extracts were analyzed by LC-MS, As a result, acetone was regarded as an optimal organic solvent for the extraction of prodigiosin from culture broth. The amount of prodigiosin extracted with acetone was almost the same as that extracted with ethanol. Moreover, the processing time could be reduced by using acetone because it has a lower boiling point than ethanol. Considering these results, we think that acetone was a very useful extraction agent for the extraction of prodigiosin as a biological control agent. [PE10050 and PN07020] 2010-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/6182 Title: Mutant selection of Hahella chejuensis KCTC 2396 and statistical optimization of medium components for prodigiosin yield-up Authors: Kim, Sung Jin; Lee, Yoo Kyung; Yim, Joung Han; Lee, Hong Kum Abstract: Prodigiosin is a natural red pigment with algicidal activity against Cochlodinium polykrikoides, a major harmful red-tide microalga. To increase the yield of prodigiosin, a mutant of Hahella chejuenesis KCTC 2396, assigned M3349, was developed by an antibiotic mutagenesis using chloramphenicol. When cultured in sucrose-based Marine Broth medium (SMB), M3349 could produce prodigiosin at 1.628±0.06 g/L, while wild type producing at 0.658±0.12 g/L under the same conditions. To increase the yield of prodigiosin production by M3349, significant medium components were determined using a two-level Plackett-Burman statistical design technique. Among fourteen components included in SMB medium, NaCl, Na2SiO3, MgCl2, H3BO3, Na2HPO4, Na2SO4, and CaCl2 were determined to be important for prodigiosin production. The medium formulation was finally optimized using a Box-Behnken design as follows: sucrose 10.0, peptone 8.0, yeast extract 2.0, NaCl 10.0, Na2SO4 12, CaCl2 1.8, MgCl2 0.7 g/L;and H3BO3 22.0, Na2HPO4 20.0, Na2SiO3 8.0 mg/L. The predicted maximum yield of prodigiosin in the optimized medium was 2.43 g/L by the Box-Behnken design, while the practical production was 2.60±0.176 g/L, which was 3.9 times higher than wild type with SMB Medium (0.658 g/L). 2008-01-01T00:00:00Z