https://repository.kopri.re.kr/handle/201206/5128 2026-04-29T18:15:03Z https://repository.kopri.re.kr/handle/201206/10626 Title: Biological Activity of Chemical Constituents Isolated from Strain Chlamydomonas sp. KSF108 (Chlamydomonadaceae) Authors: Tran, Huynh Nguyen Khanh; Youn, Ui Joung; Kim, Minji; Cao, Thao Quyen; Kim, Jeong Ah; Woo, Mi Hee; Kim, Sanghee; Min, Byung Sun Abstract: This study focused on investigation of the immunosuppressive inhibitory effect through determination of IL-2 production of nine compounds (1 ？ 9) isolated from Chlamydomonas sp. KSF108. Among them, compounds 1, 5, and 6 displayed moderately inhibitory effects on IL-2 production at a concentration of 100 μM. In addition, the related ones including cytotoxic, anti-inflammatory, and anti-oxidant activities were also elucidated. 6 further displayed cytotoxic activity against the MCF-7 cell line, with an IC50 value of 17.2 μM and 4, 6 ？ 7, and 9 possessed significant DPPH radical scavenging activity, with IC50 values ranging from 3.1 to 4.4 μM. To the best of our knowledge, this is the first report on the bioactivity of isolated chemical constituents from the genus Chlamydomonas. Compounds 1 and 5 investigated for the first time in the activity of immunosuppressivity and 6 may come to serve as the most important marker in broad-spectrum activities of the secondary metabolites identified from C. sp. KSF108. 2020-03-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/10402 Title: Increased Productivity and Antifreeze Activity of Ice-binding Protein from Flavobacterium frigoris PS1 Produced using Escherichia coli as Bioreactor Authors: Kim, Eun Jae; Kim, Jung Eun; Hwang, J. S.; Kim, Il-Chan; Lee, Sung Gu; Kim, Sanghee; Lee, Jun Hyuck; Han, Se Jong Abstract: Ice-binding proteins (IBPs) inhibit the growth and recrystallization of intracellular ice, enabling polar organisms to survive at subzero temperatures. IBPs are promising materials in biomedical applications such as cryopreservation and the hypothermic storage of cells, tissues, and organs. In this study, recombinant IBP from the antarctic bacterium Flavobacterium frigoris PS1 (FfIBP) was produced by Escherichia coli used as bioreactor, to examine the feasibility of scale-up. Oxygen transfer was the most important factor influencing cell growth and FfIBP production during pilot-scale fermentation. The final yield of recombinant FfIBP produced by E. coli harboring the pET28a-FfIBP vector system was 1.6 g/L, 3.8-fold higher than that from the previously published report using pCold I-FfIBP vector system, and its thermal hysteresis activity was 2.5°C at 9.7 μM. This study demonstrates the successful pilot-scale production of FfIBP. 2019-09-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/10976 Title: Investigation of chemical compounds from Chlamydomonas sp. KSF108 (Chlamydomonadaceae) Authors: Tran, Huynh Nguyen Khanh; Kim, Jeong Ah; Youn, Ui Joung; Kim, Sanghee; Woo, Mi Hee; Min, Byung Sun Abstract: Nine compounds (1-9) including one norisoprenoid (1), one polyol-glycoside (2), three sterols (3-5), three phenols (6, 8, and 9), and one fatty acid (7) were isolated from Chlamydomonas sp. KSF108. Their chemical structures were established using NMR spectroscopic techniques and compared with published data. None of the compounds have been previously reported from the genus Chlamydomonas and they may therefore serve as chemotaxonomic markers for Chlamydomonas sp. KSF108 within the genus. 2019-04-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/10869 Title: Anti-inflammatory activity of compounds from the rhizome of Cnidium officinale Authors: Huynh, Nguyen Khanh Tran; Cao, Thao Quyen; Kim, Jeong Ah; Youn, Ui Joung; Kim, Sanghee; Woo, Mi Hee; Min, Byung Sun Abstract: Abstract Five new compounds, 9,30-dimethoxyhierochin A (1), 6-oxo-trans-neocnidilide (2), (±)-(3E)-trans-6-hydroxy-7-methoxydihydroligustilide (3), (±)-cnidiumin (4), and 6-(1-oxopentyl)-salicylic acid methyl ester (5), together with twenty known compounds (6？25), were isolated from the rhizome of Cnidium officinale. The chemical structures of new compounds were established by NMR spectroscopic techniques, mass spectrometry, Mosher's method, and CD spectrum. Their anti-inflammatory activities were evaluated against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophage RAW 264.7 cells. Compounds 7, 13, and 14 showed inhibitory effects with IC50 values of 5.1, 24.5, and 27.8 lM, respectively. In addition, compounds 7, 13, and 14 reduced LPS-induced inducible nitric oxide synthase (iNOS) expression and cyclooxygenase-2 (COX-2) protein in a concentration-dependent manner. 2018-10-01T00:00:00Z