https://repository.kopri.re.kr/handle/201206/5301 2026-04-21T07:41:32Z https://repository.kopri.re.kr/handle/201206/12300 Title: Lipid formation by Arctic microalga Chlamydomonas sp. KNF0008 at low temperature Authors: Kim, Eun Jae; Jung, Woongsic; Lim, Suyoun; Kim, Sanghee; Choi, Han-Gu; Han, Se Jong Abstract: In the present study, we introduce a new lipid-producing microalga, Chlamydomonas sp. KNF0008, collected from the Arctic. This strain was capable of growing at temperatures ranging from 4 oC to 20 oC, and the highest cell density was measured at 15 oC and 100 μmol photons m2 s1 light intensity under continuous shaking and external aeration. KNF0008 showed the elevated accumulation of lipid bodies under nitrogen-deficient conditions, rather than under nitrogen-sufficient conditions. Fatty acid production of KNF0008 was 4.2-fold (104 mg L1) higher than that of C. reinhardtii CC-125 at 15 oC in Bold’s Basal Medium. The dominant fatty acids were C16:0, C16:4, C18:1, and C18:3, and unsaturated fatty acids (65.69%) were higher than saturated fatty acids (13.65%) at 15oC. These results suggested that Arctic Chlamydomonas sp. KNF0008 could possibly be utilized for production of biodiesel during periods of cold weather because of its psychrophilic characteristics. 2019-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/9152 Title: Production of bioenergy using cold resistance polar organisms Authors: Han, Se Jong 2016-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/7162 Title: Studies of arctic Chlamydomonas sp. KNM0029C for biodiesel production Authors: Kim, Eun Jae; Han, Se Jong; Choi, Han-Gu; Kim, Sanghee; Jung, Woongsic Abstract: Biodiesel has attracted interest because this fuel not only can replace the conventional diesel as petroleum fuel but has a low effect to environmental issue. Biodiesel produced by polar microalgae could be used in cold countries, because it has advantages that clogging can be prevented in the fuel supply due to high content of polyunsaturated fatty acids which lower the melting point of biodiesel. In this study, we visualized intracellular lipid formations of Arctic KNM0029C, and analyzed contents of fatty acids by using GC. In addition, to enhance the cell mass and lipid production of KNM0029C, we optimized medium component of Tris-acetate-phosphate (TAP). In conclusion, main fatty acids contents were C18:1, C18:2, C18:3, and C20:2 at 4°C and its maximum lipid production reached 178.6mg L-1 which was 2.3-fold higher than that of C. reinhardtii CC-125 as mesophilic strain. When KNM0029C was cultured in optimized TAP medium, total cell numbers and lipid production were increased to ~35% and ~10%, respectively. The results of the present study could potentially contribute toward large-scale lipid production at low temperatures. 2016-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/6580 Title: Pilot-scale production of the antifreeze protein from Antarctic bacterium Flavobacterium frigoris PS1 by recombinant Escherichia coli with a cold shock induction system Authors: Kim, Eun Jae; Han, Se Jong; Lee, Sung Gu; Lee, Jun Hyuck Abstract: Antifreeze proteins (AFP) inhibit growth and recrystallization of ice, and permit organisms to survive in cold environments. The AFP from an Antarctic bacterium, Flavobacterium frigoris PS1, FfIBP (Flavobacterium frigoris ice-binding protein), was produced in E. coli using a cold shock induction system. The culture temperature was shifted from 37°C to 15°C and a 20 L culture scale was used. The final weights of dried cell and FfIBP were estimated to be 126 g and 8.4 g, respectively. The thermal hysteresis (TH) activity (1.53°C) of the produced FfIBP was 3.6-fold higher than that of the LeIBP (Leucosporidium ice-binding protein) produced in Picha. The current study demonstrates that large-scale production of FfIBP was successful and the result could be extended to further application studies using recombinant AFPs. 2015-01-01T00:00:00Z