https://repository.kopri.re.kr/handle/201206/5515 2026-04-14T08:00:53Z https://repository.kopri.re.kr/handle/201206/5968 Title: Isolation of Protease-Producing Arctic Marine Bacteria Authors: Sung, Ki Cheol; Lee, Yoo Kyung; Yim, Joung Han; Park, Kyu Jin; Lee, Hong Kum; Chung, Hosung Abstract: We isolated and identified three protease-producing bacteria that had inhabited the region around the Korean Arctic Research Station Dasan located at Ny-Alesund, Svalbard, Norway (79oN, 12oE). Biofilms were collected from the surface of a floating pier and from dead brown algae in a tide pool near the seashore. The biofilm samples were transported to the Korea Polar Research Institute (KOPRI) under frozen conditions, diluted in sterilized seawater, and cultured on Zobell agar plates with 1% skim milk at 10oC. Three clear zone forming colonies were selected as protease-producing bacteria. Phylogenetic analysis based on 16S rDNA sequences showed that these three stains shared high sequence similarities with Pseudoalteromonas elyakovii, Exiguobacterium oxidotolerum and Pseudomonas jessenii, respectively. We expect these Arctic bacteria may be used to develop new varieties of protease that are active at low temperatures. 2005-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/7577 Title: Diversity of Marine Bacterial Communities in Arctic Biofilms using Cultivation and Cultivation-independent methods Authors: Cho, Hyun Hee; Cho, Kyeung Hee; Lee, Yoo Kyung; Lee, Hong Kum Abstract: Bacterial diversity in Arctic marine biofilms was analyzed through cultivation and cultivation – independent methods. We collected biofilms formed on a floating-pier and a plastic cable under seawater which were located around the Korea Arctic Research Station Dasan at Ny-Alesund, Svalbard, Norway (79ON, 12OE). A total of 95 strains were cultured, and 22 species were finally identified by phylogenetic analysis on the basis of 16S rDNA sequences. They belonged to γ-proteobacteria, Gram high GC+, Cytophaga-Flavobacterium group. The phylogenetic analysis of 110 clones which were randomly selected from 16S rDNA clone libraries, showed that about 54% of the clones belonged to Cytophaga-Flavobacterium group. The most frequent clone was an uncultured Cytophaga sp. DGGE of the partially amplified 16S rDNA was performed and DNA fragments in the most intense bands were sequenced. The majority of retrieved sequences were associated with an uncultured bacterium and an uncultured Cytophaga sp. Diversity of cultured bacteria was quite different from bacterial diversity obtained from cultivation-independent methods. 2005-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/5967 Title: Phylogenetic Analysis of Culturable Arctic Bacteria Authors: Kang, Sung-Ho; Kim, Yeadong; Lee, Yoo Kyung; Lee, Hong Kum; Cho, Kyeung Hee; Kim, Hyo Won Abstract: We isolated and identified culturable Arctic bacteria that had inhabited soils around the Korean Arctic Research Station Dasan located at Ny-Alsund, Svalbard, Norway (79oN, 12oE). The collected soils were diluted in distilled water;the diluted soil-water was spread on 3 M petri-films at Dasan Station. The petri-films were transported to the laboratory at KORDI, and cultured at 4oC. Colonies grown on the petri-films were subsequently cultured on nutrient agar plates at 4oC every 7 days. The pure colonies were inoculated into nutrient liquid media, genomic DNA was extracted, and phylogenetic analysis was performed on the basis of 16S rDNA sequences. A total of 227 strains of bacteria were isolated. Among them, 16S rDNA sequences of 185 strains were identical with those of known strains isolated in this study, and 42 strains were finally identified. Phylogenetic analysis using 16S rDNA indicated that the 30 strains belonged to Pseudomonas, 7 strains to Arthrobacter, two strains to Flavobacterium, and the remaining to Achromobacter, Pedobacter, and Psychrobacter. Among the 42 strains, 14 bacteria produced protease: they were 6 strains of Pseudomonas, 4 strains of Arthrobacter, an Achromobacter strain, 2 strains of Flavobacterium, and a Pedobacter strain. We expect these Arctic bacteria can be used for screening to develop new industrial enzymes that are active at low temperatures. 2004-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/5964 Title: Metal Concentrations in Some Brown Seaweeds from Kongsfjorden on Spitsbergen, Svalbard Islands Authors: Kim, Ji Hee; Ji, Jung Youn; Choi, Heeseon J.; Ahn, In-Young; Chung, Hosung Abstract: Concentrations of Al, As, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, Zn were determined in four arctic brown algae (Laminaria saccharina, L. digitata, Alaria esculenta, Desmarestia aculeata) in an attempt to examine for their metal accumulation capacity and also to assess their contamination levels. Macroalgae were collected from shallow subtidal waters ( 2004-01-01T00:00:00Z