https://repository.kopri.re.kr/handle/201206/15862 Sat, 11 Apr 2026 14:06:37 GMT 2026-04-11T14:06:37Z https://repository.kopri.re.kr/handle/201206/16465 Title: A novel multitarget compound mitigates amyloid plaques, synaptic defcits, and neuroinfammation in Alzheimer’s disease models Authors: Lee, Yeongyeong; Han, Sukmin; Lee, Jeongmi; Cho, Yongeun; Kim, Jun Sik; Jeon, Yeji; Cho, Heewon; Yoo, Heejin; Byun, Yujung; Kim, Tai Kyoung; Hong, Ju-Mi; Kim, Hyunwook; Park, Sang Yoon; Yim, Joung Han; Kim, Sung Hyun; Jo, Dong Gyu Abstract: Alzheimer’s disease (AD) is characterized by progressive cognitive decline, amyloid plaque accumulation, synaptic dys function, and neuroinflammation. This study reports the therapeutic potential of (S)-4-amino-5,5-difluoro-N′-methyl-N′phenylpentanehydrazide hydrochloride (RA-058HM), a novel compound, in ameliorating these pathological features of AD in the 5xFAD mouse model. RA-058HM was administered orally for 8 weeks, and its multi-target effects ？ including relief from neuroinflammation, normalization of synaptic transmission, reduction of amyloidogenesis (plaque and soluble oligomers, as well as BACE1 levels), and rescue of cognitive function―were evaluated. To our knowledge, RA-058HM is the first compound to demonstrate simultaneous modulation of these key pathways in the 5xFAD model, highlighting its potential as a comprehensive disease-modifying therapy for AD. Behavioural tests revealed marked improvements in spatial and recognition memory in RA-058HM-treated 5xFAD mice, suggesting a reversal of cognitive deficits. At the molecular level, RA-058HM treatment reduced amyloidogenesis, as evidenced by decreased levels of amyloid precursor protein (APP) and β-secretase (BACE1) in the hippocampus, accompanied by reduced plaque formation, as detected by Thioflavin-S staining. Furthermore, synaptic transmission was restored to near-normal levels in RA-058HM-treated neurons, indicating that RA-058HM effectively rescues synaptic deficits without altering synaptic protein levels of PSD95 and synaptophysin. In addition, treatment of RA-058HM downregulated hippocampal levels of the NLRP3 inflammasome, TNF-α, and GFAP, suggesting a decrease in neuroinflammatory signaling and a modulation of glial activity. Restoration of mitochondrial motility in hippocampal neurons further suggests that RA-058HM may improve cellular energy dynamics. Collectively, these findings indicate that RA-058HM has multifaceted effects on AD pathology, targeting amyloid accumulation, synaptic transmission, neuroinflammation, and mitochondrial function. This study highlights RA-058HM as a promising candidate for AD therapy and underscores the potential of multi-targeted approaches in addressing the complex mechanisms underlying AD progression. Tue, 01 Jul 2025 00:00:00 GMT https://repository.kopri.re.kr/handle/201206/16465 2025-07-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/16062 Title: Trends and Challenges in Plant Cryopreservation Research: A Meta-Analysis of Cryoprotective Agent Development and Research Focus Authors: Kang, Pilsung; Kim, Sung Jin; Park, Ha Ju; Han, Se Jong; Kim, Il-Chan; Lee, Hyoungseok; Yim, Joung Han Abstract: The stable long-term preservation of plant cells is crucial for biopharmaceuticals and food security. Therefore, the long-term cryopreservation of plant cells using a cryoprotective agent (CPA) is a crucial area of study. However, research on low-toxicity CPAs remains limited. We analyzed 1643 abstracts related to plant-cryopreservation (PCP) research published from 1967 to May 2023, spanning 56 years, from academic citation databases, with the search conducted in May 2023. Grouping these abstracts by five-year intervals revealed an increase in PCP papers until 2015, followed by a decline in the 2020s. In order to confirm the declining trend, we performed text-mining analysis using the Latent Dirichlet Allocation (LDA) algorithm, which identifies underlying topics across diverse documents to aid decision-making and classified the abstracts into three distinct topics: Topic 1, "Seed bank"; Topic 2, "Physiology"; and Topic 3, "Cryopreservation protocol". The decline, particularly in "Cryopreservation protocol" research, is an important observation in this study. At the same time, this decrease may be due to the limited scope of Topic 3. However, we expect improvements with the development of new CPAs. This expectation is based on numerous ongoing studies focused on developing new CPAs for the cryopreservation of various animal and medical cell lines, with particular attention on polysaccharides as components that could reduce the required concentrations of existing CPAs. Sat, 01 Feb 2025 00:00:00 GMT https://repository.kopri.re.kr/handle/201206/16062 2025-02-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/16185 Title: Anti-Neuroinflammatory Effects of Prenylated Indole Alkaloids from the Antarctic Fungus Aspergillus sp. Strain SF-7367 Authors: Liu Zhiming; Yoon Chi-Su; Cao Thao Quyen; Lee Hwan; Kim, Il-Chan; Yim, Joung Han; Sohn Jae Hak; Lee Dong-Sung; Oh Hyuncheol Abstract: Inflammation has always been considered a trigger or consequence of neurodegenerative diseases, and the inhibition of inflammation in the central nervous system can effectively protect nerve cells. Several studies have indicated that various natural products inhibit neuroinflammation. Among these, Antarctic fungal metabolites have pharmacological activities and a developmental value. Therefore, this study aimed to evaluate the anti-neuroinflammatory activity of an Antarctic fungus belonging to Aspergillus (strain SF-7367). Secondary metabolites of SF-7367 were isolated using high-performance liquid chromatography followed by validation of their anti-inflammatory effects in lipopolysaccharide-stimulated BV2 microglia and RAW264.7 macrophages. Chemical analysis of metabolites from the fungal strain revealed five known compounds: epideoxybrevianamide E (1), brevianamide V/W (2), brevianamide K (3), brevianamide Q (4), and brevianamide R (5). Among these compounds, brevianamide K showed significant anti-inflammatory activity against both cell types. Results of Western blotting and molecular docking showed that brevianamide K could regulate the activation of nuclear factor kappa-light-chain-enhancer of activated B cell (NF-kappa B) signaling. This indicates that brevianamide K present in Aspergillus sp. (strain SF-7367) can inhibit inflammatory responses by reducing lipopolysaccharide-induced nuclear translocation of NF-kappa B (p65). These findings suggest that Aspergillus sp. (strain SF-7367) and brevianamide K are candidate agents for treating neurodegenerative diseases. Wed, 01 Jan 2025 00:00:00 GMT https://repository.kopri.re.kr/handle/201206/16185 2025-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/16025 Title: N 5-((perfluorophenyl)amino)glutamine regulates BACE1, tau phosphorylation, synaptic function, and neuroinflammation in Alzheimer's disease models Authors: Kim, Jun-Sik; Cho, Yongeun; Lee, Jeongmi; Cho, Heewon; Han, Sukmin; Lee, Yeongyeong; Jeon, Yeji; Kim, Tai Kyoung; Hong, Ju-Mi; Im, Jeonghyeong; Chae, Minshik; Lee, Yujeong; Kim, Hyunwook; Park, Sang Yoon; Kim, Sung Hyun; Yim, Joung Han; Jo, Dong-Gyu Abstract: Alzheimer's disease (AD) is the most common type of dementia. Its incidence is rising rapidly as the global population ages, leading to a significant social and economic burden. AD involves complex pathologies, including amyloid plaque accumulation, synaptic dysfunction, and neuroinflammation. This study explores the therapeutic potential of N5-((perfluorophenyl)amino)glutamine (RA-PF), a derivative of gamma-glutamyl-N'-(2hydroxyphenyl)hydrazide (Ramalin), a compound with antioxidant and anti-inflammatory properties. Administration of RA-PF to 5xFAD mice decreases BACE1, reduces A & tcedil;3 plaque deposition, inhibits microglial activation, restores synaptic transmission, and improves mitochondrial motility, leading to the recovery of cognitive function. Additionally, RA-PF treatment in 3xTg-AD mice alleviates anxiety-like behaviors, tau phosphorylation via inactivating GSK-3 & tcedil;3, and BACE1 expression. Further transcriptomic analysis reveals RA-PF treatment in AD mice models recovers phagosome, These findings suggest that RA-PF effectively targets multiple aspects of AD pathology, offering a novel multi-target Wed, 01 Jan 2025 00:00:00 GMT https://repository.kopri.re.kr/handle/201206/16025 2025-01-01T00:00:00Z