https://repository.kopri.re.kr/handle/201206/5154 Thu, 16 Apr 2026 02:49:31 GMT 2026-04-16T02:49:31Z https://repository.kopri.re.kr/handle/201206/7448 Title: Succession of bacterial community structure during the early stage of biofilm development in the Antarctic marine environment Authors: Cho, Kyeung Hee; Hwang, Kyuin; Lee, Yung Mi; Lee, Hong Kum; Hong, Soon Gyu; Kim, Mincheol; Kim, Eun Hye Abstract: Compared to planktonic bacterial populations, biofilms have distinct bacterial community structures and play important ecological roles in various aquatic environments. Despite their ecological importance in nature, bacterial community structure and its succession during biofilm development in the Antarctic marine environment have not been elucidated. In this study, the succession of bacterial community, particularly during the early stage of biofilm development, in the Antarctic marine environment was investigated by pyrosequencing of the 16S rRNA gene. Overall phyla distribution in biofilms differed considerably from surrounding seawater. Relative abundance of Gammaproteobacteria and Bacteroidetes which accounted for 78.9？88.3% of bacterial community changed drastically during biofilm succession. Gammaproteobacteria became more abundant with proceeding succession (75.7% on day 4) and decreased to 46.1% on day 7. The relative abundance of Bacteroidetes showed opposite trend to Gammaproteobacteria, decreasing from the early days to the intermediate days and becoming more abundant in the later days. There were striking differences in the composition of major OTUs (>1%) among samples during the early stages of biofilm formation. Gammaproteobacterial species increased until day 4, while members of Bacteroidetes, the most dominant group on day 1, decreased until day 4 and then increased again. Interestingly, Pseudoalteromonas prydzensis was predominant, accounting for up to 67.4% of the biofilm bacterial community indicating its important roles in the biofilm development. Fri, 01 Jan 2016 00:00:00 GMT https://repository.kopri.re.kr/handle/201206/7448 2016-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/7465 Title: Bacterial communities in Antarctic lichens Authors: Park, Chae Haeng; Jeong, Gajin; Hong, Soon Gyu; Kim, Kyung Mo; Kim, Ok-Sun Abstract: So far, many studies to survey the bacterial communities in lichen thalli from diverse geographical areas have shown that Alphaproteobacteria is a predominating bacterial class in most lichens. In this study, we analyzed bacterial communities in several Antarctic lichens with different growth form and substrates. Bacterial community composition in fruticose and foliose lichens, Cladonia, Umbilicaria, Usnea, and crustose lichens, Buelia granulosa, Amandinea coniops, and Ochrolechia parella from King George Island, Antarctica was analyzed by pyrosequencing of bacterial 16S rRNA gene. Results showed that Proteobacteria, Acidobacteria, Actinobacteria, and Bacteroidetes were predominant phyla. The predominant bacterial class in most of the samples was Alphaproteobacteria, which have also been found previously in other lichen species. Acetobacteriaceae of the order Rhodospiralles in Alphaproteobacteria was the most abundant bacterial family in Antarctic lichens. The LAR1 lineage of the order Rhizobiales, a putative N-fixer which has been frequently observed in lichens from temperate area, was detected only from few samples at low frequency. It is expected that other bacterial taxa are working as N-fixer in Antacrtic lichens. From the PcoA analysis of fastUniFrac distance matrix, it was proposed that microbial community structures in Antarctic lichens were affected by host species, growth form, and substrates. Fri, 01 Jan 2016 00:00:00 GMT https://repository.kopri.re.kr/handle/201206/7465 2016-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/7273 Title: Algal and Fungal Diversity in Antarctic Lichens Authors: Kim, Kyung Mo; Hong, Soon Gyu; Jeong, Gajin; Kim, Ok-Sun; Arve Elvebakk; Park, Chae Haeng Abstract: The composition of lichen ecosystems except mycobiont and photobiont have not been evaluated intensively. In addition, recent studies to identify algal genotypes have raised questions about the specific relationship between mycobiont and photobiont. In the current study, we analyzed algal and lichen-associated fungal community structure in lichen species from King George Island, Antarctica by pyrosequencing of eukaryotic LSU and algal ITS domains of the nuclear rRNA gene. The sequencing results of LSU and ITS regions indicated that each lichen thallus contained diverse algal species. The major algal operational taxonomic unit (OTU) defined at a 99% similarity cut-off of LSU sequences accounted for 78.7% to 100% of the total algal community in each sample. In several cases, the major OTUs defined by LSU sequences were represented by two closely related OTUs defined by 98% sequence similarity of ITS domain. The results of LSU sequences indicated that lichen-associated fungi belonged to the Arthoniomycetes, Eurotiomycetes, Lecanoromycetes, Leotiomycetes, and Sordariomycetes of the Ascomycota, and Tremellomycetes and Cystobasidiomycetes of the Basidiomycota. The composition of major photobiont species and lichen-associated fungal community were mostly related with the mycobiont species. The contribution of growth forms or substrates on composition of photobiont and lichen-associated fungi were not evident. Thu, 01 Jan 2015 00:00:00 GMT https://repository.kopri.re.kr/handle/201206/7273 2015-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/6714 Title: Transposon mutagenesis of Psychrobacter cryohalolentis PAMC 21807 by tri-parental conjugation Authors: Lee, Yoo Kyung; Jeong, Hyun-Jeong; Lee, Hyoungseok; Hong, Soon Gyu; Cho, Jang-Cheon; Lee, Hong Kum Tue, 01 Jan 2013 00:00:00 GMT https://repository.kopri.re.kr/handle/201206/6714 2013-01-01T00:00:00Z